Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   AB0R67_RS05805 Genome accession   NZ_CP160237
Coordinates   1119846..1123361 (+) Length   1171 a.a.
NCBI ID   WP_335446213.1    Uniprot ID   -
Organism   Bacillus thuringiensis strain JJ1216     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1114846..1128361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB0R67_RS05765 (AB0R67_05770) - 1115008..1115502 (-) 495 WP_065228454.1 DUF6584 family protein -
  AB0R67_RS05770 (AB0R67_05775) cspA 1115787..1115990 (+) 204 WP_000301518.1 RNA chaperone/antiterminator CspA -
  AB0R67_RS05775 (AB0R67_05780) - 1116188..1116391 (-) 204 WP_000356913.1 hypothetical protein -
  AB0R67_RS05780 (AB0R67_05785) - 1116740..1116925 (-) 186 WP_000391373.1 hypothetical protein -
  AB0R67_RS05785 (AB0R67_05790) - 1117211..1117792 (+) 582 WP_071714131.1 competence protein ComK -
  AB0R67_RS05790 (AB0R67_05795) - 1117864..1118163 (-) 300 WP_080476084.1 hypothetical protein -
  AB0R67_RS05795 (AB0R67_05800) - 1118516..1119109 (+) 594 WP_071714132.1 TVP38/TMEM64 family protein -
  AB0R67_RS05800 (AB0R67_05805) lepB 1119166..1119729 (+) 564 WP_000751897.1 signal peptidase I -
  AB0R67_RS05805 (AB0R67_05810) addB 1119846..1123361 (+) 3516 WP_335446213.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  AB0R67_RS05810 (AB0R67_05815) addA 1123358..1127083 (+) 3726 WP_335446214.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  AB0R67_RS05815 (AB0R67_05820) - 1127107..1127385 (+) 279 WP_090975801.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  AB0R67_RS05820 (AB0R67_05825) gerPF 1127522..1127737 (-) 216 WP_001141566.1 spore germination protein GerPF -
  AB0R67_RS05825 (AB0R67_05830) - 1127780..1128166 (-) 387 WP_098284191.1 spore germination protein GerPE -

Sequence

Protein


Download         Length: 1171 a.a.        Molecular weight: 134038.23 Da        Isoelectric Point: 5.2113

>NTDB_id=1020367 AB0R67_RS05805 WP_335446213.1 1119846..1123361(+) (addB) [Bacillus thuringiensis strain JJ1216]
MSLRFVIGRAGSGKSTLCLREVQEELKQRPRGKTILYLVPEQMTFQTQQALIGSEDVRGSIRAQVFSFSRLAWKVLQEVG
GASRLHIDEAGVHMLLRKIVESRKDGLSVFQKAAEQNGFFEHLGSMIAEFKRYNVTPSNVYEMWQQLDAHSSSAEQKLLA
NKVYDLQLLYDDFERALIGKYLDSEDYLQLLVEKLPQSEHVNGAEIYIDGFHSFSPQELEIVRQLMICGAKVTITLTIDE
KTLAQPVNELDLFYETTLTYEKIKQVAREEKIEIEKTIPLMEQPRFHSPALAHLEAHYEARPNEKFNGEASVTISTAANL
RAEVEGVAREIRKLVADEKYRYRDIAVLLRNGESYYDVMRTLFTDYNIPHFIDEKRPMSHHPLVECIRSALEIISGNWRY
DAVFRCVKTELLYPLDVRKEAMREEMDEFENYCLAYGVQGKRWTAEDPWMYRRYRSLDDTNGMITDSEREMEEKINRLRD
VVRTPVIRMQKRLKRAGTVMQMCEAVYLFLEELDVPKKLEELRIRAEESGDFLFATDHEQVWEEVISLLDTFVEMLGEEK
MSLSMFTDVMSTGLEALQFANIPPSLDQVLIANIDHSRLSDVRATFIIGVNEGVIPAAPMDEGMLSDEEREVLGAAGIEL
APTTRQTLLEEQFVMYQMVTRASEKLYISCPLADEEGKTLLASSFIKKIKRMFPNVKDSFITNDVNDLSRSEQISYVATP
EVTLSYVMQQLQTWKRYGFEGNLDFWWDVYNFYVTSDEWKQKSSRVLSSLFYRNRAQKLSTAVSRDLYGDTIKGSVSRME
LFNRCAYAHFAQHGLSLRERDIFKLDAPDIGELFHAALKKIADKLLRENRTWADLSIKECEHLSVLVIEEIAPLLQRQIL
LSSNRHFYLKQKLQQIIFRTSIILREHAKSSGFVPVDLEVPFGMGGTGSLPPMEFSLPNGVKMEVVGRIDRVDKAEDENG
TFLRIIDYKSSSKALDLTEVYYGLALQMLTYLDVVTSNAQTWMKKGHAASPAGVLYFHIHNPIVEMKGDASEAEIEKEIL
KKFKMKGLVLGDADVVRLMDNKLSTGSSDIISAGLKKDGSFSARSSIASEQEFNVLQKYVHHTFENIGKDITEGVIDIAP
YKMGNKAACTFCNFKSVCQFDESLEDNQFRSLKDMKDSEAMEKIREEVGGE

Nucleotide


Download         Length: 3516 bp        

>NTDB_id=1020367 AB0R67_RS05805 WP_335446213.1 1119846..1123361(+) (addB) [Bacillus thuringiensis strain JJ1216]
ATGTCACTTCGATTTGTGATTGGTAGAGCGGGAAGTGGAAAAAGTACACTTTGTTTACGCGAAGTGCAAGAAGAGTTAAA
ACAGCGCCCGAGAGGGAAAACAATATTATATCTTGTGCCAGAACAGATGACATTCCAGACGCAGCAGGCGTTAATTGGAA
GTGAGGATGTAAGAGGTTCTATTCGGGCACAAGTTTTTAGTTTTTCACGATTAGCGTGGAAGGTGCTGCAAGAAGTTGGC
GGAGCGAGTCGTCTTCATATTGATGAAGCTGGTGTACATATGTTACTCCGTAAAATTGTAGAGTCTCGTAAAGATGGATT
ATCGGTGTTCCAAAAAGCAGCGGAGCAAAACGGTTTCTTTGAACATCTTGGCAGTATGATCGCGGAGTTTAAACGTTACA
ATGTAACGCCATCTAACGTATATGAAATGTGGCAACAATTAGATGCGCATAGTAGCAGTGCCGAACAAAAGTTACTAGCG
AATAAAGTGTATGATTTACAGTTACTATACGATGATTTTGAACGTGCTTTAATTGGAAAATACTTAGATTCAGAAGACTA
CCTGCAATTGCTAGTGGAAAAGCTTCCGCAGTCTGAACATGTAAATGGTGCGGAAATTTATATAGATGGATTTCATTCAT
TCTCCCCGCAAGAGTTAGAGATTGTAAGACAGCTTATGATTTGCGGAGCAAAAGTTACGATCACGTTAACGATAGATGAA
AAAACGTTAGCACAGCCAGTAAATGAACTCGATTTATTTTATGAAACGACGTTAACGTATGAAAAAATAAAACAAGTAGC
GCGTGAAGAGAAAATAGAAATTGAAAAAACAATTCCACTTATGGAACAGCCGCGTTTTCATTCTCCAGCATTAGCGCATT
TAGAAGCTCATTATGAAGCGCGTCCGAATGAAAAATTTAACGGTGAAGCGAGTGTAACGATTAGTACAGCTGCGAATTTA
CGAGCGGAGGTAGAAGGTGTTGCTCGTGAAATTCGTAAACTTGTTGCGGACGAAAAATATCGTTACCGAGATATTGCAGT
GCTTCTTCGTAATGGGGAAAGTTATTACGATGTGATGCGAACGTTATTTACAGATTACAATATCCCGCACTTCATCGATG
AAAAGCGCCCGATGTCACACCACCCATTGGTAGAATGTATTCGTTCTGCACTCGAGATTATTAGTGGGAATTGGCGTTAT
GATGCGGTGTTCCGTTGCGTGAAAACAGAGCTTTTATATCCATTAGACGTAAGAAAAGAAGCGATGCGTGAAGAAATGGA
TGAATTTGAAAATTACTGTTTAGCGTACGGTGTACAAGGAAAGAGATGGACTGCTGAAGATCCATGGATGTATCGCCGTT
ATCGTTCTCTTGATGATACGAACGGGATGATTACAGACAGTGAACGTGAAATGGAAGAGAAAATAAATAGGCTACGTGAC
GTTGTAAGAACACCTGTTATTCGCATGCAAAAAAGGTTAAAGCGTGCAGGAACAGTTATGCAAATGTGTGAAGCAGTTTA
CTTGTTTTTAGAAGAACTGGATGTTCCGAAGAAACTAGAAGAATTACGTATACGTGCAGAAGAAAGTGGAGATTTCTTAT
TTGCGACAGATCATGAACAAGTATGGGAAGAAGTAATAAGCCTTCTTGATACATTTGTAGAGATGCTTGGGGAAGAGAAA
ATGTCTCTTTCTATGTTTACTGACGTTATGTCGACAGGTCTTGAGGCACTTCAATTTGCTAACATTCCGCCGTCATTAGA
CCAAGTGTTAATTGCTAATATTGATCACTCCAGGTTATCTGATGTGAGAGCAACGTTTATTATAGGTGTAAATGAGGGTG
TCATTCCAGCAGCACCTATGGATGAAGGTATGCTTTCTGATGAGGAAAGAGAAGTTCTTGGTGCAGCAGGGATTGAATTA
GCGCCAACGACGAGACAAACTTTGTTAGAAGAACAGTTCGTTATGTATCAAATGGTAACGAGAGCATCTGAGAAGTTATA
CATTTCATGCCCGCTTGCAGATGAAGAAGGAAAGACGTTACTTGCGTCTAGCTTTATTAAGAAAATAAAAAGAATGTTCC
CTAATGTGAAAGATTCGTTTATTACGAATGATGTAAACGACTTATCACGCTCAGAACAAATTTCATATGTAGCAACGCCA
GAAGTGACGTTATCTTACGTTATGCAGCAACTACAAACGTGGAAACGATACGGATTTGAAGGGAATTTAGATTTTTGGTG
GGATGTATATAATTTCTACGTAACGTCAGATGAATGGAAACAAAAAAGTAGTCGCGTGCTATCAAGTTTATTTTACCGAA
ATCGTGCGCAGAAACTAAGTACAGCAGTAAGTAGAGATTTATACGGAGATACAATTAAAGGAAGCGTCTCTCGTATGGAA
CTATTTAATCGTTGTGCATACGCTCATTTCGCGCAGCATGGTTTGTCGCTTAGAGAGCGTGATATTTTCAAACTTGATGC
GCCAGATATCGGCGAATTATTCCATGCGGCATTAAAGAAAATTGCGGACAAGTTATTGCGTGAAAACCGCACTTGGGCGG
ATTTATCAATAAAAGAATGTGAACATCTTTCTGTTTTAGTAATAGAAGAAATTGCACCGTTATTACAACGACAAATTTTA
TTAAGTTCAAATCGTCATTTCTATTTAAAACAAAAACTACAACAAATTATTTTCCGCACGTCAATCATTCTTCGTGAACA
TGCGAAGTCGAGCGGATTCGTACCAGTTGATTTAGAGGTGCCGTTCGGTATGGGTGGTACAGGGTCACTTCCGCCAATGG
AATTCTCATTACCAAATGGTGTAAAGATGGAAGTAGTCGGTCGTATTGACCGTGTTGATAAGGCAGAAGATGAAAACGGG
ACGTTCTTACGTATTATTGATTATAAATCAAGTTCAAAAGCGTTAGATTTAACGGAAGTGTATTACGGATTAGCACTTCA
AATGTTAACGTATTTAGATGTTGTTACTTCGAATGCACAGACGTGGATGAAAAAAGGCCACGCAGCATCACCAGCTGGTG
TATTGTATTTCCATATTCATAACCCAATCGTTGAGATGAAAGGTGACGCATCTGAAGCTGAAATTGAAAAAGAAATTTTA
AAGAAATTTAAAATGAAAGGACTCGTATTAGGAGATGCTGACGTTGTTCGTTTAATGGATAACAAACTTTCAACAGGAAG
CTCAGATATTATTTCTGCTGGCCTGAAAAAAGACGGTAGCTTTAGTGCGCGTTCAAGTATTGCAAGTGAGCAAGAGTTTA
ACGTACTGCAAAAATATGTACACCATACGTTTGAAAATATTGGAAAAGATATTACAGAAGGTGTTATCGATATTGCTCCT
TATAAAATGGGAAATAAAGCAGCATGTACGTTCTGTAACTTCAAATCTGTTTGTCAATTTGATGAGTCACTTGAAGATAA
TCAGTTCCGATCGTTAAAAGATATGAAAGATAGCGAAGCGATGGAGAAAATAAGAGAGGAGGTTGGCGGAGAATGA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(303-669)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(795-1139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

49.403

100

0.494

Multiple sequence alignment