Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   ABS324_RS08960 Genome accession   NZ_CP158496
Coordinates   1738714..1741053 (+) Length   779 a.a.
NCBI ID   WP_061664892.1    Uniprot ID   A0A9X7CQ17
Organism   Bacillus cereus strain NBNZ-2162     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1733714..1746053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABS324_RS08945 (ABS324_08945) - 1734744..1735028 (+) 285 WP_000886978.1 post-transcriptional regulator -
  ABS324_RS08950 (ABS324_08950) secDF 1735298..1737562 (+) 2265 WP_001119060.1 protein translocase subunit SecDF -
  ABS324_RS08955 (ABS324_08955) - 1737719..1738612 (+) 894 WP_000409743.1 cation diffusion facilitator family transporter -
  ABS324_RS08960 (ABS324_08960) recJ 1738714..1741053 (+) 2340 WP_061664892.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  ABS324_RS08965 (ABS324_08965) - 1741101..1741613 (+) 513 WP_000346215.1 adenine phosphoribosyltransferase -
  ABS324_RS08970 (ABS324_08970) relA 1741824..1744007 (+) 2184 WP_001262813.1 GTP diphosphokinase -
  ABS324_RS08975 (ABS324_08975) - 1744024..1744464 (+) 441 WP_001266965.1 D-tyrosyl-tRNA(Tyr) deacylase -
  ABS324_RS08980 (ABS324_08980) - 1744630..1744806 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88132.66 Da        Isoelectric Point: 4.9019

>NTDB_id=1011605 ABS324_RS08960 WP_061664892.1 1738714..1741053(+) (recJ) [Bacillus cereus strain NBNZ-2162]
MLQPKTRWKEKEYNDERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGAEVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEANVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLEGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTRNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDAAPAVHLLLSDDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVENNFPPEENKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLSIDPIKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQADAILTDEDFIPITAVDVFCKVEDVTLAAIEDMQKLAPFGI
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNAEANLAELPKEKITMVYFSEEVLNKFSLESYKEKLMHASEVTHLDDQYIVLLDLPKGTDELRE
LFKVGFPARIYTLFYQENNHLFSTVPTREHFKWYYSFLSQKTPFSLRQYGEQLCRHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=1011605 ABS324_RS08960 WP_061664892.1 1738714..1741053(+) (recJ) [Bacillus cereus strain NBNZ-2162]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAATGACGAGCGAGTGAGTGAATTAGCAAGTAAATTACAGTT
ATCGCCACTTGTCGTTTCATTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCCATTCAAAATGGA
GAGCAAATATTAATATTTGGTGATTACGATGCGGACGGAGTAAGTAGTACGACGGTTTTATATCTTGCTCTTCAAGAATT
AGGTGCAGAAGTAGAATTTTACATTCCAAATCGTTTTACAGAAGGGTATGGACCGAACGAAGAAGCGTTTCGTTGGGCGC
ATAGCGCAGGGTTCTCACTGATTATTACTGTCGACACTGGCATCGCAGCAGTACATGAAGCAAATGTAGCGAAAGAGCTT
GGAATAGATCTAATTATTACAGATCACCATGAGCCACCGCCAGAATTGCCAGAAGCACTTGCGATTATACATCCGAAATT
AGAAGGTGGCGTATATCCGTTTCACTATTTAGCTGGTGTAGGTGTTGCGTTTAAAGTTGCGCATGCACTATTAGGCCGTG
TACCAGAACATTTATTAGAGATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCATGGTGAAAATAGGTTGCTA
GTGAAACGCGGTTTAAAACATATGCGTATGACGAGAAATATTGGTTTGAAGGCATTGTTTAAAGTTGCTAACGTTTCGCA
AAGTGAAATTACCGAAGAGAGTATTGGTTTCTCAATTGCACCTCGTATTAATGCGGTCGGGCGTTTAGAAGATGCAGCGC
CGGCTGTACACCTTTTATTATCAGATGATCCAGAGGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGCTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAGGCTATCGCTGAAGTGGAAAATAACTTCCCACCAGAAGAAAATAAAGT
ATTAGTACTTGCAAAAGAAGGCTGGAACCCAGGGGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CGACGATTGTATTAAGCATTGATCCTATAAAAGAAACAGCAAAAGGCTCAGCACGTAGTATTGCAGGATTTGATTTGTTT
GCAAATTTATCAGATTGTAGAGAGTTATTACCACACTTCGGAGGGCATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGTCGCCGCTTAAATGAACAAGCAGATGCAATTTTAACAGACGAAGATTTTATTCCAATTACAG
CAGTTGATGTTTTTTGCAAAGTAGAGGATGTAACGTTAGCGGCAATTGAAGATATGCAAAAACTAGCCCCGTTTGGTATT
GGAAATCCGAAACCACGTATTGCAGTAAAAGATGCAGAGTTAGAAAGCATTCGCGCAATCGGATCAGATGGCTCTCATTT
AAAAATGGCTCTTCGTGATGGACAAGCAACACTGGATACAATTGGATTTGGTTTTGGTGCATATGCGAAAGAAATCTCTC
CAGTTGCAAAAGTATCTGTAATAGGAGAAGCGTCTATTAATGAATGGAATAACTTTAAGAAGCCACAACTAATGGTACAA
GATATTGCTGTAGAGGCGTGGCAATTATTTGATTGGCGTAGTATGCGTAATGCAGAAGCAAATTTAGCAGAACTTCCGAA
AGAAAAAATAACAATGGTGTATTTTTCTGAAGAGGTATTGAATAAATTTTCTTTAGAGAGCTATAAAGAAAAGCTCATGC
ATGCTTCAGAAGTAACGCATTTAGATGATCAATATATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGTGAG
CTATTTAAGGTAGGATTCCCAGCGCGAATTTATACGCTATTTTATCAAGAGAATAACCACTTATTTAGTACAGTTCCGAC
GAGAGAACACTTTAAATGGTACTATTCTTTCTTAAGCCAAAAAACACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCGTCATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAATTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGTTAGGGTAA

Domains

Predicted by InterproScan.

DHH family (DHH)

(82-226)

RecJ OB domain (RecJ_OB)

(455-561)

DHHA1 domain (DHHA1)

(315-439)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.196

99.358

0.519

Multiple sequence alignment