Detailed information NTG1004479

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: E0F34_RS07950
• Genome accession: NZ_LR536843
• Coordinates: 1466646..1469042 (-)
• Length: 798 a.a.
• NCBI ID: WP_044791468.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1461646..1474042

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F34_RS07930 (SAMEA104035599_01502)	stkP	1461661..1463640 (-)	1980	WP_044793148.1	serine/threonine-protein kinase StkP	Regulator
E0F34_RS07935 (SAMEA104035599_01503)	-	1463637..1464377 (-)	741	WP_000406247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
E0F34_RS07940 (SAMEA104035599_01504)	rsmB	1464392..1465705 (-)	1314	WP_001809725.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
E0F34_RS07945 (SAMEA104035599_01505)	fmt	1465698..1466633 (-)	936	WP_061649868.1	methionyl-tRNA formyltransferase	-
E0F34_RS07950 (SAMEA104035599_01506)	priA	1466646..1469042 (-)	2397	WP_044791468.1	primosomal protein N'	Machinery gene
E0F34_RS07955 (SAMEA104035599_01507)	rpoZ	1469108..1469422 (-)	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
E0F34_RS07960 (SAMEA104035599_01508)	gmk	1469447..1470073 (-)	627	WP_000775037.1	guanylate kinase	-
E0F34_RS07965 (SAMEA104035599_01509)	-	1470203..1471816 (-)	1614	WP_000404940.1	ribonuclease Y	-
E0F34_RS07970 (SAMEA104035599_01510)	-	1472007..1472261 (-)	255	WP_000924721.1	Txe/YoeB family addiction module toxin	-
E0F34_RS07975 (SAMEA104035599_01511)	-	1472265..1472519 (-)	255	WP_000388572.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E0F34_RS07980 (SAMEA104035599_01512)	-	1472757..1473854 (-)	1098	WP_050122521.1	nucleotidyltransferase	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 89965.74 Da
• Isoelectric Point: 6.2622

>NTDB_id=1004479 E0F34_RS07950 WP_044791468.1 1466646..1469042(-) (priA) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGGAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Nucleotide

• Length: 2397 bp

>NTDB_id=1004479 E0F34_RS07950 WP_044791468.1 1466646..1469042(-) (priA) [Streptococcus pneumoniae strain GPSC55 substr. ST3774 isolate b04a6400-1f66-11e7-b93e-3c4a9275d6c8]
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGGAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATCTCTATCCTCAAGGCCAT
GCTTCCGGGATTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTGCGCC
TGTTTGGTTCAGAAGATTCGCTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGGTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCTCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTGGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGCAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
TGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGGTGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGTTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTCTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATCCGCAGCACTATGCTATTCGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAGGATGAGCTGGGGCCGACCCTCAACCAGG
TTTTAGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-109)

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• Helicase conserved C-terminal domain (Helicase_C) (572-656)

• Type III restriction enzyme, res subunit (ResIII) (260-424)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.182	100	0.47