Detailed information NTG1003759

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: E0F32_RS08440
• Genome accession: NZ_LR216050
• Coordinates: 1579016..1581412 (-)
• Length: 798 a.a.
• NCBI ID: WP_130885959.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1574016..1586412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E0F32_RS08420 (SAMEA3431333_01581)	stkP	1574031..1576010 (-)	1980	WP_000614540.1	serine/threonine-protein kinase StkP	Regulator
E0F32_RS08425 (SAMEA3431333_01582)	-	1576007..1576747 (-)	741	WP_130885958.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
E0F32_RS08430 (SAMEA3431333_01583)	rsmB	1576762..1578075 (-)	1314	WP_001812475.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
E0F32_RS08435 (SAMEA3431333_01584)	fmt	1578068..1579003 (-)	936	WP_000163704.1	methionyl-tRNA formyltransferase	-
E0F32_RS08440 (SAMEA3431333_01585)	priA	1579016..1581412 (-)	2397	WP_130885959.1	primosomal protein N'	Machinery gene
E0F32_RS08445 (SAMEA3431333_01586)	rpoZ	1581478..1581792 (-)	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
E0F32_RS08450 (SAMEA3431333_01587)	gmk	1581817..1582443 (-)	627	WP_000775037.1	guanylate kinase	-
E0F32_RS08455 (SAMEA3431333_01588)	-	1582573..1584186 (-)	1614	WP_000404940.1	ribonuclease Y	-
E0F32_RS08460 (SAMEA3431333_01589)	-	1584377..1584631 (-)	255	WP_000924721.1	Txe/YoeB family addiction module toxin	-
E0F32_RS08465 (SAMEA3431333_01590)	-	1584635..1584889 (-)	255	WP_000388572.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
E0F32_RS08470 (SAMEA3431333_01591)	-	1585127..1586224 (-)	1098	WP_130885960.1	nucleotidyltransferase	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 90085.81 Da
• Isoelectric Point: 6.1582

>NTDB_id=1003759 E0F32_RS08440 WP_130885959.1 1579016..1581412(-) (priA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFDSEDSLAFSSLDLGKQAEMMRLTRK
GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Nucleotide

• Length: 2397 bp

>NTDB_id=1003759 E0F32_RS08440 WP_130885959.1 1579016..1581412(-) (priA) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGGAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATCTCTATCCTCAAGGCCAT
GCTTCCGGGATTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTACGCC
TGTTTGATTCAGAAGATTCGCTAGCCTTTTCTTCTCTGGATTTAGGTAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGGTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCTCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTAGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCGACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGTAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
CGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGATGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTCTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTCTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATTCGCAGCACTATGCTATTCGATTTGCTAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGG
TCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Domains

Predicted by InterProScan.

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• Helicase conserved C-terminal domain (Helicase_C) (572-656)

• Type III restriction enzyme, res subunit (ResIII) (260-424)

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-109)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	45.936	100	0.467