Detailed information NTG0100324

Bioinformatically predicted

Overview

• Name: comYH
• Type: Machinery gene
• Locus tag: AACH30_RS08740
• Genome accession: NZ_AP027243
• Coordinates: 1775986..1776921 (-)
• Length: 311 a.a.
• NCBI ID: WP_003133316.1
• Uniprot ID: A0A1I4GTA7
• Organism: Lactococcus garvieae strain L21-68
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1770986..1781921

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AACH30_RS08715 (LG21E68_17220)	-	1771034..1771894 (-)	861	WP_003133325.1	S1 RNA-binding domain-containing protein	-
AACH30_RS08720 (LG21E68_17230)	frr	1772002..1772559 (-)	558	WP_003133324.1	ribosome recycling factor	-
AACH30_RS08725 (LG21E68_17240)	pyrH	1772589..1773305 (-)	717	WP_003133322.1	UMP kinase	-
AACH30_RS08730 (LG21E68_17250)	-	1773396..1774589 (-)	1194	WP_003133320.1	acetate kinase	-
AACH30_RS08735 (LG21E68_17260)	-	1774708..1775901 (-)	1194	WP_265149676.1	acetate kinase	-
AACH30_RS08740 (LG21E68_17270)	comYH	1775986..1776921 (-)	936	WP_003133316.1	class I SAM-dependent methyltransferase	Machinery gene
AACH30_RS08745 (LG21E68_17280)	-	1776967..1777197 (-)	231	WP_003133314.1	DUF3165 family protein	-
AACH30_RS08750 (LG21E68_17290)	typA	1777272..1779113 (-)	1842	WP_003133313.1	translational GTPase TypA	-
AACH30_RS08755 (LG21E68_17300)	-	1779434..1779646 (+)	213	WP_003133311.1	YqgQ family protein	-
AACH30_RS08760 (LG21E68_17310)	-	1779639..1780604 (+)	966	WP_003133309.1	ROK family glucokinase	-
AACH30_RS08765 (LG21E68_17320)	-	1780624..1781004 (+)	381	WP_003133308.1	rhodanese-like domain-containing protein	-
AACH30_RS08770 (LG21E68_17330)	-	1781044..1781490 (-)	447	WP_003133306.1	Dps family protein	-

Sequence

Protein

• Length: 311 a.a.
• Molecular weight: 35124.17 Da
• Isoelectric Point: 4.6693

>NTDB_id=100324 AACH30_RS08740 WP_003133316.1 1775986..1776921(-) (comYH) [Lactococcus garvieae strain L21-68]
MDIEKIEKAFGLVLENIMAIQAELLTDFYDAFVEQNAAYLGALEFASLTKENNDKVRSMNLTKSEWQKLFQFVLLQGSRV
APMQANHNLTPDSIGFIFNFIIEELHKERKIRVLEFGSGTGNLAETLLVHMQKEVDYVGFEVDDLLLDLSASMSEVMGTE
ASFLQIDAVKPQPLESVDVVMSDLPVGYYPDDDTARHFQVHDKSEHTYVHHLMIEQSFKYLKESGFALFLAPDNLLTSAQ
SEFLKAWIKEHAHVAAVITLPSSLFKGAGKSIYLLSKNQTNTPTFVYPLADLADRSILQEFMSEFVKNVKI

Nucleotide

• Length: 936 bp

>NTDB_id=100324 AACH30_RS08740 WP_003133316.1 1775986..1776921(-) (comYH) [Lactococcus garvieae strain L21-68]
ATGGATATAGAGAAAATCGAAAAAGCCTTTGGATTGGTTCTTGAAAATATTATGGCAATTCAAGCGGAGCTCTTGACGGA
TTTTTATGATGCTTTTGTGGAACAAAATGCAGCTTACTTAGGCGCTTTAGAGTTTGCATCTCTGACGAAGGAAAATAATG
ATAAGGTTCGTTCAATGAACCTCACGAAAAGTGAGTGGCAAAAGCTGTTTCAGTTTGTCTTGCTTCAAGGGTCACGTGTC
GCACCGATGCAAGCAAATCATAATTTAACACCAGATTCTATTGGGTTTATCTTTAATTTCATCATTGAAGAGTTGCACAA
AGAAAGAAAGATTCGTGTACTAGAATTTGGCTCAGGAACAGGGAATTTAGCGGAAACGCTCTTGGTTCATATGCAAAAAG
AAGTAGACTATGTTGGTTTTGAAGTAGATGACCTCTTGTTGGATTTGTCTGCTTCAATGTCAGAAGTCATGGGAACAGAA
GCAAGTTTTCTCCAAATTGACGCCGTGAAACCACAGCCCTTGGAGTCTGTAGATGTTGTGATGAGTGATCTCCCGGTTGG
TTATTATCCAGACGATGACACTGCTCGCCATTTTCAAGTGCATGACAAATCAGAACATACCTATGTGCACCACTTAATGA
TTGAGCAATCCTTTAAGTATCTGAAGGAGAGTGGTTTTGCGCTCTTCTTAGCTCCAGATAATCTTTTGACAAGTGCACAG
TCAGAATTTTTGAAGGCTTGGATTAAAGAACATGCACATGTTGCTGCAGTCATTACATTGCCTTCGTCACTCTTTAAAGG
TGCAGGAAAATCGATTTATTTATTAAGTAAAAACCAAACAAATACACCAACTTTTGTTTACCCCTTGGCTGATTTAGCAG
ACCGTTCTATTTTGCAAGAATTTATGTCTGAGTTTGTGAAAAATGTCAAGATTTGA

Domains

Predicted by InterproScan.

• N-6 DNA Methylase (N6_Mtase) (132-286)

3D structure

• AlphaFold DB: A0A1I4GTA7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYH	Streptococcus mutans UA140	49.677	99.678	0.495
comYH	Streptococcus mutans UA159	49.355	99.678	0.492