Detailed information NTG1003093

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EL254_RS01945
• Genome accession: NZ_LR134512
• Coordinates: 320013..322403 (+)
• Length: 796 a.a.
• NCBI ID: WP_000101528.1
• Uniprot ID: Q8E1N9
• Organism: Streptococcus agalactiae strain NCTC13949
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 315013..327403

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL254_RS01915 (NCTC13949_00372)	-	315638..316378 (+)	741	WP_001217938.1	ABC transporter permease	-
EL254_RS01920 (NCTC13949_00373)	-	316375..317460 (+)	1086	WP_000791349.1	sensor histidine kinase	-
EL254_RS01925 (NCTC13949_00374)	-	317462..318052 (+)	591	WP_000772050.1	DNA-binding response regulator	-
EL254_RS01930 (NCTC13949_00375)	-	318102..318806 (+)	705	WP_001168922.1	TMEM175 family protein	-
EL254_RS01935 (NCTC13949_00376)	gmk	318973..319602 (+)	630	WP_000003861.1	guanylate kinase	-
EL254_RS01940 (NCTC13949_00377)	rpoZ	319625..319939 (+)	315	WP_000979239.1	DNA-directed RNA polymerase subunit omega	-
EL254_RS01945 (NCTC13949_00378)	priA	320013..322403 (+)	2391	WP_000101528.1	primosomal protein N'	Machinery gene
EL254_RS01950 (NCTC13949_00379)	fmt	322450..323385 (+)	936	WP_000163893.1	methionyl-tRNA formyltransferase	-
EL254_RS01955 (NCTC13949_00380)	rsmB	323375..324697 (+)	1323	WP_001262604.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
EL254_RS01960 (NCTC13949_00381)	-	324735..325472 (+)	738	WP_000406233.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-

Sequence

Protein

• Length: 796 a.a.
• Molecular weight: 91170.95 Da
• Isoelectric Point: 8.6523

>NTDB_id=1003093 EL254_RS01945 WP_000101528.1 320013..322403(+) (priA) [Streptococcus agalactiae strain NCTC13949]
MARKLAQVIVDIPLMQTDKPFSYAIPKDLEDLVQVGVRVHVPFGRGNRLLQGFVVGFRDDDELETKDIAEVLDFEPVLNQ
EQLDLADQMRHTVFSYKISILKSMLPSLLNSQYDKLLLATDTLPSEDREDLFGHKTEIVFSSLSSQDAKKAGRLIQKGFI
EVQYLAKDKKTIKTEKIYKINRTLLEKSQIAARAKKRLELKEFLLENPQPGRLTALNKQFSSPVVNFFREEGIIEVIEKE
ASRSDNYFKGILKTDFLDLNQEQAKVVKIVVDQIGKEQNKPFLLEGITGSGKTEVYLHIIDNVLKLGKTAIVLVPEISLT
PQMTNRFISRFGKQVAIMHSGLSEGEKFDEWRKIKSGQAKVVVGARSAIFAPLENIGAIIIDEEHESTYKQESNPRYHAR
DVALLRAEYYKAVLLMGSATPSIESRARASRDVYKFLELKHRANPKARIPQVEIIDFRNFIGQQEVSNFTSYLLDKIRDR
LDKKEQVVLMLNRRGYSSFIMCRDCGYVDQCPNCDISLTLHMATKTMNCHYCGFEKPIPRTCPNCNSKSISYYGTGTQKA
YEELLKVIPDAKILRMDVDTTRQKGGHESILKRFGNHEADILLGTQMIAKGLDFPNVTLVGVLNADTSLNLPDFRSSERT
FQLLTQVAGRAGRAEKEGEVVIQTYNPNHYAIQLAQKQDFEAFYQYEMNIRRQLGYPPYYFTVGLTLSHKDEEWLIRKSY
EVLSLLKQGFSDKVKLLGPTPKPIARTHNLYHYQIIIKYRFEDNLELVLNRLLDMTQDKENRDLRLAIDHEPQNMM

Nucleotide

• Length: 2391 bp

>NTDB_id=1003093 EL254_RS01945 WP_000101528.1 320013..322403(+) (priA) [Streptococcus agalactiae strain NCTC13949]
ATGGCAAGAAAATTAGCACAGGTTATTGTTGATATTCCTCTGATGCAGACAGATAAACCATTTTCGTATGCCATTCCTAA
AGATTTGGAAGATTTGGTACAAGTAGGAGTACGCGTTCATGTTCCTTTTGGAAGAGGAAATCGTCTTTTGCAAGGCTTTG
TTGTCGGTTTCAGAGATGATGATGAATTAGAGACTAAGGATATTGCGGAGGTCCTAGATTTTGAACCTGTTTTAAATCAG
GAACAACTAGACTTAGCTGATCAGATGCGCCATACTGTCTTTTCTTATAAAATATCTATTTTGAAATCAATGTTACCAAG
TCTTTTGAATTCGCAATATGATAAACTCTTGTTAGCAACAGACACATTACCATCAGAAGATAGAGAAGATCTATTTGGTC
ATAAAACGGAAATTGTCTTTTCAAGTTTGTCTTCTCAAGATGCCAAAAAAGCAGGCAGATTAATTCAAAAAGGTTTCATA
GAAGTTCAATATTTAGCCAAAGATAAGAAAACAATAAAAACAGAAAAGATTTACAAAATCAACAGAACTCTGTTAGAAAA
GAGCCAAATAGCAGCAAGAGCGAAAAAGAGACTGGAGTTGAAAGAATTCCTGTTAGAAAATCCTCAACCAGGGCGCCTCA
CAGCTTTAAACAAACAATTTTCATCTCCTGTGGTTAATTTTTTCAGAGAAGAAGGAATCATTGAAGTCATTGAAAAAGAA
GCTAGTAGATCTGATAATTATTTTAAAGGAATCCTAAAAACGGATTTTTTGGATTTAAATCAGGAACAAGCTAAAGTAGT
TAAAATAGTTGTTGACCAAATTGGTAAGGAACAGAATAAACCCTTCTTGTTGGAGGGGATTACAGGGTCTGGTAAGACAG
AGGTATATCTTCATATCATTGATAACGTTTTAAAGTTGGGAAAAACAGCGATTGTTCTCGTTCCAGAAATTTCTTTAACT
CCCCAGATGACGAATCGTTTTATTTCTCGTTTTGGAAAGCAAGTTGCTATTATGCACTCGGGCTTGTCAGAAGGTGAAAA
ATTTGATGAGTGGCGAAAGATAAAATCAGGTCAAGCTAAAGTTGTCGTTGGAGCTCGTTCAGCAATTTTTGCTCCTTTGG
AAAATATCGGAGCCATCATTATAGATGAGGAACATGAATCAACTTATAAGCAAGAATCTAACCCAAGGTATCATGCGCGC
GATGTGGCCTTGCTACGTGCAGAATATTATAAAGCTGTGTTACTAATGGGATCAGCAACTCCAAGTATCGAAAGCCGAGC
TAGAGCTAGTCGGGATGTTTATAAATTTTTAGAATTAAAACATCGTGCTAATCCTAAAGCACGTATTCCTCAGGTTGAAA
TTATTGATTTTAGAAATTTTATTGGTCAACAAGAAGTTAGTAATTTCACATCATATTTACTAGATAAAATCCGAGATAGA
TTAGATAAGAAAGAGCAAGTGGTTCTTATGCTCAATCGTAGAGGTTATTCTAGTTTTATTATGTGTCGAGACTGCGGCTA
CGTTGATCAATGTCCTAATTGTGACATTTCTCTGACTTTACATATGGCTACAAAGACCATGAATTGTCATTATTGTGGGT
TTGAAAAGCCTATTCCAAGAACATGTCCAAATTGCAATAGTAAAAGTATCTCTTACTATGGTACTGGAACCCAAAAAGCT
TATGAAGAACTTTTGAAAGTTATCCCAGACGCAAAGATTTTACGGATGGATGTTGATACGACAAGGCAAAAAGGCGGTCA
TGAGTCTATTTTGAAGCGTTTCGGAAATCATGAAGCTGATATTTTATTGGGAACACAGATGATTGCTAAGGGATTGGATT
TTCCAAATGTTACTTTAGTTGGAGTACTAAATGCAGATACTTCTCTGAATTTACCTGATTTTCGATCATCAGAGCGAACT
TTTCAGCTATTAACTCAAGTGGCTGGACGTGCTGGGCGTGCTGAAAAAGAAGGAGAGGTTGTCATTCAAACTTATAATCC
TAATCATTATGCTATTCAATTAGCCCAAAAGCAAGATTTTGAAGCTTTTTATCAATATGAAATGAATATCCGTCGACAAC
TTGGTTATCCTCCTTATTATTTTACAGTTGGTTTAACATTATCGCATAAAGATGAAGAGTGGTTAATACGAAAATCTTAT
GAAGTATTAAGTTTATTGAAGCAAGGCTTTAGTGATAAAGTAAAGTTGTTAGGTCCAACGCCAAAACCAATTGCAAGAAC
ACATAACCTTTATCACTACCAAATTATTATCAAGTATCGATTTGAGGACAACCTTGAACTTGTTTTGAATCGCTTATTAG
ACATGACTCAAGACAAGGAGAATAGAGATCTTCGCTTAGCAATAGACCACGAACCGCAAAATATGATGTAG

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (698-792)

• Type III restriction enzyme, res subunit (ResIII) (258-422)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-106)

• Helicase conserved C-terminal domain (Helicase_C) (571-654)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (511-537)

3D structure

• AlphaFold DB: Q8E1N9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.906	100	0.476