Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   ECRM13514_RS21410 Genome accession   NZ_CP006027
Coordinates   4162203..4162670 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O145:H28 str. RM13514     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4157203..4167670
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECRM13514_RS21360 (ECRM13514_4275) rplP 4157313..4157723 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  ECRM13514_RS21365 (ECRM13514_4276) rpsC 4157736..4158437 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  ECRM13514_RS21370 (ECRM13514_4277) rplV 4158455..4158787 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  ECRM13514_RS21375 rpsS 4158802..4159080 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  ECRM13514_RS21380 (ECRM13514_4279) rplB 4159097..4159918 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  ECRM13514_RS21385 (ECRM13514_4280) rplW 4159936..4160238 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  ECRM13514_RS21390 (ECRM13514_4281) rplD 4160235..4160840 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  ECRM13514_RS21395 (ECRM13514_4282) rplC 4160851..4161480 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  ECRM13514_RS21400 (ECRM13514_4283) rpsJ 4161513..4161824 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  ECRM13514_RS21410 (ECRM13514_4284) pilD 4162203..4162670 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  ECRM13514_RS21415 (ECRM13514_4285) bfr 4162667..4163143 (-) 477 WP_000675515.1 bacterioferritin -
  ECRM13514_RS21420 (ECRM13514_4286) bfd 4163216..4163410 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  ECRM13514_RS21425 (ECRM13514_4287) tuf 4163593..4164777 (-) 1185 WP_000031783.1 elongation factor Tu -
  ECRM13514_RS21430 (ECRM13514_4288) fusA 4164848..4166962 (-) 2115 WP_000124700.1 elongation factor G -
  ECRM13514_RS21435 (ECRM13514_4289) rpsG 4167059..4167529 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=100069 ECRM13514_RS21410 WP_000381746.1 4162203..4162670(+) (pilD) [Escherichia coli O145:H28 str. RM13514]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=100069 ECRM13514_RS21410 WP_000381746.1 4162203..4162670(+) (pilD) [Escherichia coli O145:H28 str. RM13514]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCTTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment