Detailed information NTG1000529

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: EL096_RS05875
• Genome accession: NZ_LR134294
• Coordinates: 1121450..1123846 (-)
• Length: 798 a.a.
• NCBI ID: WP_001162116.1
• Uniprot ID: -
• Organism: Streptococcus pneumoniae strain NCTC12977
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1116450..1128846

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL096_RS05855 (NCTC12977_01146)	stkP	1116465..1118444 (-)	1980	WP_050221291.1	serine/threonine-protein kinase StkP	Regulator
EL096_RS05860 (NCTC12977_01147)	-	1118441..1119181 (-)	741	WP_000406247.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
EL096_RS05865 (NCTC12977_01148)	rsmB	1119196..1120509 (-)	1314	WP_001812782.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
EL096_RS05870 (NCTC12977_01149)	fmt	1120502..1121437 (-)	936	WP_050221290.1	methionyl-tRNA formyltransferase	-
EL096_RS05875 (NCTC12977_01150)	priA	1121450..1123846 (-)	2397	WP_001162116.1	primosomal protein N'	Machinery gene
EL096_RS05880 (NCTC12977_01151)	rpoZ	1123912..1124226 (-)	315	WP_000979235.1	DNA-directed RNA polymerase subunit omega	-
EL096_RS05885 (NCTC12977_01152)	gmk	1124251..1124877 (-)	627	WP_000775037.1	guanylate kinase	-
EL096_RS05890 (NCTC12977_01153)	-	1125007..1126620 (-)	1614	WP_000404940.1	ribonuclease Y	-

Sequence

Protein

• Length: 798 a.a.
• Molecular weight: 90012.80 Da
• Isoelectric Point: 6.3647

>NTDB_id=1000529 EL096_RS05875 WP_001162116.1 1121450..1123846(-) (priA) [Streptococcus pneumoniae strain NCTC12977]
MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKGNRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPV
LTPEQLWLAEELRKSVFSYKISILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLASLLESYSREQVNFFVDQGAVTIV
QKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSSIGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEIS
LTPQMTERFIARFGDKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYKQDSNPRYH
AREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLATIPEVQVIDFRDYIGQNETSNFTPPLLEAIQ
DRLVKKEQVVLMLNRRGYSSFVMCRECGTVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQ
KAYDELAELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGVLNADTALNLPDFRSSE
RTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDYEGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKR
AYEVMNILRSGLSETSPILGPTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL

Nucleotide

• Length: 2397 bp

>NTDB_id=1000529 EL096_RS05875 WP_001162116.1 1121450..1123846(-) (priA) [Streptococcus pneumoniae strain NCTC12977]
ATGGCCCTAGCTAAGATTATCGTAGATGTGCCCTTGATGCAGACGGACCAGCCCTATAGTTACAGGATTCCGGAGGAATT
TGAGGGAATGCTGGAAGTTGGGATGCGGGTTCATGTGCCTTTTGGTAAGGGTAATCGCCTGATTCAAGGGATTGTTCTTG
GTTTGAAGTCCCAATCAGATGGAGAAGAGATGGAGCAAGATTTAAAAGATATTGCAGAGGTGCTGGATTTTTCTCCTGTT
CTCACGCCAGAACAACTCTGGCTGGCTGAGGAGTTACGCAAATCTGTCTTCTCCTACAAAATCTCTATCCTCAAGGCCAT
GCTTCCAGGGTTTCTGAATTCTAGCTATGACAAGATTCTCTATCCTCTGGAAGGCTTGAGTCAGGAAGAACGAGTACGCC
TGTTTGGTTCAGAAGATTCGCTAGCCTTTTCTTCACTAGACCTTGCCAAGCAAGCTGAAATGATGCGTTTGACTAGAAAA
GGCCTGCTTGGTCTGGAATATCAGGCAGTCGATCAAAAGAAGGTCAAGACCCAGTCTTGGTATGAGGTTGACCATGCCCA
ATTAGAAGGTGTTGAGATTTCTACACGTGCCAAGAAAAAGTTGGAACTGAGAGATTATCTGCTGTCTCATCCAGAGAGCG
CTTCCTTGGCTAGCTTGTTAGAGTCCTACTCGCGAGAGCAAGTCAACTTCTTTGTGGATCAAGGTGCTGTTACCATAGTC
CAAAAGGAAGTTCAACGCTCGGCTGCTTATTTTGAAGGAATTGAAGCAAGTAGACCTTTGGAGTTAAATCCAGAACAAAG
ACAGGCGCGTGATGCGGTTGTGAGTTCTATTGGCAGTTCTCAACCTCCCTTTCTACTTCAAGGGATTACAGGAAGTGGGA
AGACCGAGGTTTACTTGCAGATTATCCAAGGTGCCCTGGATAAGGGTAAGACAGCTATTTTGCTGGTACCTGAGATTTCC
TTGACTCCTCAGATGACCGAGCGTTTTATAGCACGTTTTGGGGACAAGGTAGCCATTCTTCACTCAGGATTATCCAATGG
CGAAAAGTATGATGAATGGCGCAAGGTGGAGCGTGGCGATGCCCAAGTTGTTGTTGGTGCCAGATCTGCTATCTTTGCTC
CTCTGAAAAATCTGGGTGTCATGATTATTGATGAAGAGCATGAAGCGGCTTATAAGCAGGACAGCAATCCCCGTTACCAT
GCCAGAGAGGTAGCTATTTTACGGGCTCAGTATAATCAAGCAACTCTGGTACTTGGATCTGCAACGCCGAGCTTAGAGAG
CCGGGCACGTGCTGGCAAAGGCGTCTATCAACACTTACGTCTAACCCAACGTGCCAATCCTTTGGCTACAATCCCTGAGG
TTCAAGTGATTGACTTTCGAGACTATATCGGACAAAATGAGACGTCAAACTTTACGCCTCCTTTGCTAGAGGCTATCCAA
GACCGTCTGGTTAAAAAAGAGCAGGTGGTTCTCATGCTCAATCGCCGTGGTTATTCTAGCTTTGTCATGTGTCGGGAGTG
TGGGACGGTGGATACTTGTCCCAACTGCGATATTTCTCTGACCTTGCATATGGATACCAAGACTATGAACTGCCATTATT
GTGGTTTTTCGAAGGATATTCCTCAGGTCTGTCCTAACTGTAAGAGCCGCAGTATTCGTTACTATGGGACGGGAACTCAG
AAGGCTTATGATGAGCTAGCAGAACTCTTTCCCCAAGCTCGCATTTTGAGGATGGATGTGGATACGACTCGAAAGAAAGG
CAGTCACCAAGCCTTGCTTGACCAGTTTGGGCGAGGGGAAGCGGATATTTTACTTGGTACTCAGATGATTGCAAAGGGTT
TGGATTTTCCCAATGTTACCCTAGTCGGAGTTCTAAATGCGGATACGGCCTTGAATCTGCCTGATTTCCGTTCTTCTGAG
AGAACCTTCCAGCTCTTGACTCAGGTGGCAGGTCGAGCAGGTCGGGCTGAAAAAGCAGGTCAAGTCTTGATACAGTCCTA
CAATTCGCAGCACTATGCTATTCGATTTGCCAAGGATCAAGATTACGAAGGCTTTTATGCCTATGAAATGGGAATCAGAC
GACAACTCGGCTATCCACCTTACTATTTCACGATTGGTATTACCCTTTCTCACAAGAAAGAAGAAGAGGTTTTCAAACGT
GCCTATGAAGTCATGAACATTTTGCGGTCAGGCTTGTCAGAGACCAGTCCCATTCTGGGGCCAACGCCAAAACCGATTGC
CCGTACCCACAACCTCTATCATTACCAGATTTTAATTAAATACCGTTTAGAAGATGAGCTGGGGCCGACCCTCAACCAGG
TCTTGGCCTTGACTCAAGAACGGGAAAATAGTGAGCTCCGTCTCAGCATTGACCATGAGCCGCAGCAATTTTTATAA

Domains

Predicted by InterProScan.

• Primosomal protein N C-terminal domain (PriA_C) (700-794)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (513-538)

• Helicase conserved C-terminal domain (Helicase_C) (572-656)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (5-109)

• Type III restriction enzyme, res subunit (ResIII) (260-424)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Bacillus subtilis subsp. subtilis str. 168	46.182	100	0.47