Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU20_RS14640 Genome accession   NZ_CP155963
Coordinates   3291570..3292700 (-) Length   376 a.a.
NCBI ID   WP_005990431.1    Uniprot ID   Q8P759
Organism   Xanthomonas campestris pv. raphani strain BR66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3286570..3297700
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU20_RS14620 (ABFU20_14575) - 3287682..3288221 (+) 540 WP_014508432.1 DNA-3-methyladenine glycosylase I -
  ABFU20_RS14625 (ABFU20_14580) - 3288218..3289480 (+) 1263 WP_014508433.1 hypothetical protein -
  ABFU20_RS14630 (ABFU20_14585) - 3289703..3290602 (+) 900 WP_014508434.1 DUF72 domain-containing protein -
  ABFU20_RS14635 (ABFU20_14590) - 3290616..3291290 (-) 675 WP_014508435.1 YitT family protein -
  ABFU20_RS14640 (ABFU20_14595) pilU 3291570..3292700 (-) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU20_RS14645 (ABFU20_14600) pilT 3292823..3293860 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU20_RS14650 (ABFU20_14605) - 3294147..3294830 (+) 684 WP_014508436.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU20_RS14655 (ABFU20_14610) proC 3294871..3295731 (+) 861 WP_014508437.1 pyrroline-5-carboxylate reductase -
  ABFU20_RS14660 (ABFU20_14615) - 3296271..3296693 (+) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU20_RS14665 (ABFU20_14620) - 3296837..3297427 (+) 591 WP_080565893.1 M23 family metallopeptidase -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41996.26 Da        Isoelectric Point: 6.8287

>NTDB_id=1000360 ABFU20_RS14640 WP_005990431.1 3291570..3292700(-) (pilU) [Xanthomonas campestris pv. raphani strain BR66]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVHGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVEELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRGRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGLDGVEIAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=1000360 ABFU20_RS14640 WP_005990431.1 3291570..3292700(-) (pilU) [Xanthomonas campestris pv. raphani strain BR66]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGATCTGTTCATCACCTCTGGCAT
GCCGCCGGCGATCAAGGTGCATGGCAAGATCAGCCCGATCACCCAGACGCCGCTGACCGCGCAGCAGAGTCGCGACCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGTCCGGG
GTGGGGCGGTTCCGTGTGAGTTGCTTCTATCAGCGCAACCAGGTGGGCATGGTGTTGCGGCGGATCGAGACGCGCATTCC
CACCGTGGAAGAGCTGAGCCTGCCGCCGGTGATCAAGACACTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CTACCGGCACCGGCAAGTCGACCTCGCTGGCGGCGATGATCGGCTACCGCAACCAGAATTCCACCGGGCACATCATCACC
ATCGAAGACCCGATCGAGTTCGTGCACAAGCACGAGGGTTGCATTATCACCCAGCGCGAAGTCGGCATCGATACCGATAG
CTGGGAAAATGCGCTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAAGTGCGGACCCGCGAAGGCA
TGGACCATGCCATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCATGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCGGAAGATCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAACCTCAAGGGTGTGGT
GGCGCAGCAGTTGATCCCGACCCCGGATGGCCGCGGCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GATCAGAGCCTGTTCGAGCTGTACCAGGCGGGCGAGATCAGCTACGAAGACGCACTGCGCTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTCTCGCAAGGCGGCGATGCCAAGACGCTGTCGCAGGGGCTCGATGGCGTGGAGATTGCCG
AGGTTCGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8P759

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

67.575

97.606

0.66
  pilU Acinetobacter baylyi ADP1

63.866

94.947

0.606
  pilU Vibrio cholerae strain A1552

53.652

94.681

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Pseudomonas aeruginosa PAK

41.194

89.096

0.367

Multiple sequence alignment