 ICEberg ID | 162_IME |
Name  | IMESp2907  |
| Family | - |
| Organism | Streptococcus pyogenes iB21 |
| Size (bp) | 12.6 kb |
| GC content [Genome] (%) | |
| Insertion site | Internal site of orf8 from ICESp2905 and orf10 from ICESp1108 |
| Function | erm(TR) (macrolide, lincosamide, streptogramin) |
| Species that IME can be transferred to | Streptococcus pyogenes; Streptococcus agalactiae |
| Nucleotide Sequence | FR691055 (complete IME sequence in this contig) |
| Replicon | - |
| Coordinates | 6733..19348 |
| Putative oriT region | - |
| Putative relaxase | coordinates: 12685..14325; Family: MOBQ |
The Interaction Network among ICE/IME/CIME/plasmid | ||||||
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| Detailed Informatioin of the Interaction Network | ||||||
| # | IME | Inter_Ele [Type] | Methods | Donors | Recipients | Exper_Ref |
| 1 | IMESp2907 | ICESp2905 [ICE] |  in cis | - | - | - |
| 2 | IMESp2907 | ICESagTR7 [ICE] | in cis | Streptococcus agalactiae SagTR7 | Streptococcus agalactiae 1357RF | 26679245 |
| This is an interactioin derived from experimental literature |
| This is a putative interactioin |
The graph information of IMESp2907 components from FR691055 | |||||
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| Complete gene list of IMESp2907 from FR691055 | |||||
| # | Gene | Coordinates [+/-], size (bp) | Product | *Reannotation | |
| 1 | - | 1838..2122 [+], 285 | hypothetical protein | ||
| 2 | - | 2137..2988 [+], 852 | Replication initiator protein A | ||
| 3 | - | 2985..3836 [+], 852 | DNA replication protein | ||
| 4 | - | 3833..4318 [+], 486 | hypothetical protein | ||
| 5 | - | 4315..6099 [+], 1785 | TraG/TraD/VirD4 family bacterial conjugation protein | ||
| 6 | - | 6153..6266 [+], 114 | hypothetical protein | ||
| 7 | - | 6266..6577 [+], 312 | hypothetical protein | ||
| 8 | - | 6581..6739 [+], 159 | hypothetical protein | ||
| 9 | - | 6857..7075 [+], 219 | Transcriptional regulator | ||
| 10 | - | 7079..7456 [+], 378 | hypothetical protein | ||
| 11 | - | 7866..8303 [+], 438 | MarR bacterial repressor protein | ||
| 12 | - | 8293..9600 [+], 1308 | MATE domain protein | ||
| 13 | - | 9700..9813 [+], 114 | hypothetical protein | ||
| 14 | erm(TR) | 10305..11036 [+], 732 | erythromycin resistance methylase | AR | |
| 15 | - | 11253..11777 [+], 525 | Spectinomycin phosphotransferase | ||
| 16 | - | 11823..11963 [+], 141 | hypothetical protein | ||
| 17 | - | 12021..12335 [+], 315 | hypothetical protein | ||
| 18 | - | 12685..14325 [+], 1641 | MobA/MobL family protein | Relaxase | |
| 19 | - | 14398..14571 [+], 174 | hypothetical protein | ||
| 20 | - | 14755..15282 [+], 528 | hypothetical protein | ||
| 21 | - | 15485..16516 [+], 1032 | Nuclease of restriction endonuclease | ||
| 22 | - | 16582..17310 [+], 729 | hypothetical protein | ||
| 23 | - | 17465..17689 [+], 225 | hypothetical protein | ||
| 24 | - | 17693..19339 [+], 1647 | TndX-like transposase subfamily | Integrase | |
| 25 | - | 19446..20309 [+], 864 | hypothetical protein | ||
| 26 | - | 20327..20590 [+], 264 | hypothetical protein | ||
| 27 | - | 20594..20983 [+], 390 | hypothetical protein | ||
| 28 | - | 20895..23324 [+], 2430 | Type IV secretory pathway VirB4 component | ||
 Flanking regions |
 Gene may contribute to site-specific recombination |
 Gene may play role in conjugative transfer |
 Gene may be involved in adaptative function |
| (1) Guedon G; Libante V; Coluzzi C; Payot S; Leblond-Bourget N (2017). The Obscure World of Integrative and Mobilizable Elements, Highly Widespread Elements that Pirate Bacterial Conjugative Systems. Genes (Basel). 8(11). [PudMed:29165361] |
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(2) Morici E; Simoni S; Brenciani A; Giovanetti E; Varaldo PE; Mingoia M (2017). A new mosaic integrative and conjugative element from Streptococcus agalactiae carrying resistance genes for chloramphenicol (catQ) and macrolides [mef(I) and erm(TR)]. J Antimicrob Chemother. 72(1):64-67. [PudMed:27621174]
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(3) Mingoia M; Morici E; Marini E; Brenciani A; Giovanetti E; Varaldo PE (2016). Macrolide resistance gene erm(TR) and erm(TR)-carrying genetic elements in Streptococcus agalactiae: characterization of ICESagTR7, a new composite element containing IMESp2907. J Antimicrob Chemother. 71(3):593-600. [PudMed:26679245]
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| (4) Bellanger X; Payot S; Leblond-Bourget N; Guedon G (2014). Conjugative and mobilizable genomic islands in bacteria: evolution and diversity. FEMS Microbiol Rev. 38(4):720-60. [PudMed:24372381] |
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(5) Giovanetti E; Brenciani A; Tiberi E; Bacciaglia A; Varaldo PE (2012). ICESp2905, the erm(TR)-tet(O) element of Streptococcus pyogenes, is formed by two independent integrative and conjugative elements. Antimicrob Agents Chemother. 56(1):591-4. [PudMed:21986826]
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(6) Brenciani A; Tiberi E; Bacciaglia A; Petrelli D; Varaldo PE; Giovanetti E (2011). Two distinct genetic elements are responsible for erm(TR)-mediated erythromycin resistance in tetracycline-susceptible and tetracycline-resistant strains of Streptococcus pyogenes. Antimicrob Agents Chemother. 55(5):2106-12. [PudMed:21343455]
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| experimental literature |