Detailed information of T4CP

T4CP

ID   7 GenBank   FAA00040
Name   TrwB_R388 experimental UniProt ID   Q04230
Length   507 a.a. PDB ID   1E9R, 1E9S, 1GKI, 1GL6, 1GL7
Note   The T4CP TrwB_R388 ATPase activity is stimulated by ssDNA, dsDNA and the C-terminal Region of auxiliary protein TrwA_R388 [PMID:17599913].

  Protein sequence


Download         Length: 507 a.a.        Molecular weight: 56272.95 Da        Isoelectric Point: 9.8647

>FAA00040.1 TPA: TrwB protein (plasmid) [Escherichia coli]
MHPDDQRKVSAGIVIVLPLIFWITAVQKTEVLGSPKLLALWELMKLTPQKPILLLSALGGLAVGVLFVWL
LNSVGQGEFGGAPFKRFLRGTRIVSGGKLKRMTREKAKQVTVAGVPMPRDAEPRHLLVNGATGTGKSVLL
RELAYTGLLRGDRMVIVDPNGDMLSKFGRDKDIILNPYDQRTKGWSFFNEIRNDYDWQRYALSVVPRGKT
DEAEEWASYGRLLLRETAKKLALIGTPSMRELFHWTTIATFDDLRGFLEGTLAESLFAGSNEASKALTSA
RFVLSDKLPEHVTMPDGDFSIRSWLEDPNGGNLFITWREDMGPALRPLISAWVDVVCTSILSLPEEPKRR
LWLFIDELASLEKLASLADALTKGRKAGLRVVAGLQSTSQLDDVYGVKEAQTLRASFRSLVVLGGSRTDP
KTNEDMSLSLGEHEVERDRYSKNTGKHHSTGRALERVRERVVMPAEIANLPDLTAYVGFAGNRPIAKVPL
EIKQFANRQPAFVEGTI

  Protein domains


Predicted by InterproScan

Type IV secretion-system coupling protein DNA-binding domain (TrwB_AAD_bind)

(110-490)

  Protein structure


Source ID Structure
AlphaFold DB Q04230

  Reference


[1] de la Cruz F et al. (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol Rev. 34(1):18-40. [PMID:19919603]
[2] Fernández-López R et al. (2006) Dynamics of the IncW genetic backbone imply general trends in conjugative plasmid evolution. FEMS Microbiol Rev. 30(6):942-66. [PMID:17026718]
[3] Llosa M et al. (1994) Genetic organization of the conjugal DNA processing region of the IncW plasmid R388. J Mol Biol. 235(2):448-64. [PMID:8289274]
[4] Cabezón E et al. (1994) Requirements for mobilization of plasmids RSF1010 and ColE1 by the IncW plasmid R388: trwB and RP4 traG are interchangeable. J Bacteriol. 176(14):4455-8. [PMID:8021231]