Detailed information of relaxase

Relaxase

ID   8 GenBank   P07112
Name   MobA_R1162 experimental UniProt ID   P07112
Family   MOBQ PDB ID   2NS6
Length   709 a.a.
Note   (1) Tyr25 residue of the relaxase MobA_R1162 is essential for catalytic activity [PMID:17157875].
 (2) The relaxase MobA_R1162 contains two, auxiliary protein MobB_R1162-dependent type IV transport signals [PMID:17880426].
 (3) The N-terminal and C-terminal of relaxase MobA_R1162 are independently transported by the type IV pathway [PMID:17880426].

  Protein sequence


Download         Length: 709 a.a.        Molecular weight: Da        Isoelectric Point:

>sp|P07112.4|MOBA2_ECOLX RecName: Full=Mobilization protein A; AltName: Full=DNA strand transferase; Includes: RecName: Full=DNA relaxase; AltName: Full=DNA nickase; Includes: RecName: Full=DNA primase
MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESGHMPEFVERPADYWDAADLYERANGR
LFKEVEFALPVELTLDQQKALASEFAQHLTGAERLPYTLAIHAGGGENPHCHLMISERINDGIERPAAQW
FKRYNGKTPEKGGAQKTEALKPKAWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGP
NVVEMEGRGIRTDRADVALNIDTANAQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTG
RRSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGVALEAMANRDA
GFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQM
MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGK
TATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDF
IAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGM
DRGGPDFSM

  Protein domains



No domain identified.


  Protein structure


Source ID Structure
PDB 2NS6
AlphaFold DB P07112

  Reference


[1] de la Cruz F et al. (2010) Conjugative DNA metabolism in Gram-negative bacteria. FEMS Microbiol Rev. 34(1):18-40. [PMID:19919603]
[2] Meyer R (2009) The R1162 mob proteins can promote conjugative transfer from cryptic origins in the bacterial chromosome. J Bacteriol. 191(5):1574-80. [PMID:19074386]
[3] Parker C et al. (2007) The R1162 relaxase/primase contains two, type IV transport signals that require the small plasmid protein MobB. Mol Microbiol. 66(1):252-61. [PMID:17880426]
[4] Jandle S et al. (2006) Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer. J Bacteriol. 188(2):499-506. [PMID:16385040]
[5] Zhang S et al. (1997) The relaxosome protein MobC promotes conjugal plasmid mobilization by extending DNA strand separation to the nick site at the origin of transfer. Mol Microbiol. 25(3):509-16. [PMID:9302013]
[6] Brasch MA et al. (1986) Genetic organization of plasmid R1162 DNA involved in conjugative mobilization. J Bacteriol. 167(2):703-10. [PMID:3525520]