Detailed information of relaxase

Relaxase

• ID: 6
• GenBank: AAM90727
• Name: TraI_pED208
• UniProt ID: Q8KNJ9
• Family: MOB_F
• PDB ID: _
• Length: 1767 a.a.
• Note: OriT nicking and unwinding; similar to TraI of plasmid F

Protein sequence

• Length: 1767 a.a.
• Molecular weight: 193052.42 Da
• Isoelectric Point: 4.9480

>AAM90727.1 TraI (plasmid) [Salmonella enterica subsp. enterica serovar Typhi]
MLSISSIKGDAGYYSHEDNYYASGSLDSRWMGEGAEKLGLKGEVASADMDAVRQGRLPDGSDLSRMVDGV
NKHRSGYDLTFSAPKSVSVMALIGEDRRFIEAHNRAVAVVMQEVEQLVSARITQEGKTETVLTGSMVAAL
YNHDTSRDLDPQVHTHALVFNATFADEKWRSLASDTRMKTGFSENLYATKIALGNLYRSALREDIESMGF
ETVAAGKHGLWELKDVPVDIFSSRSQAIREAAGPDASAKSRDVAALDTRQAKAWADPDLLKAEWRRRLTD
EKFDIDNYISQAQARVEVSGPVVAGQGGISASGQPGIASSGEAADELVQKAVGDTISALSDKKVQFTWSE
MLAGTVSRLPSAPGLFEQARAGIEAAIEGQRLIPLDREKGIFTSDIHLLNELSVHQLARTAVAEQTVLVF
PERAQERNIPAGDAVSVLSQDKSPVAILSCRGGAQALRERTEDVAMMARSQGREVMVIAADGRSGQFLSE
SPQLAGQVMLRSQINADTVLPVQGTVIVDRAERLSLKETVLLQEKALSAGAQLIFMDTENRQGTGNALSV
LKEADIPQYRFYGTQLPEVRLVSEADKRSRYSQLAQDYVRLSAEGRDVVAQVTGTREQQQLTEIIRDTRR
EAGELGREQVTLRVLEPVWLDSKTRHQRDNYRPGMVMEQWDAEKKTMTRHTIDRVAEATNSLVLQGEDGT
RLTLKVTQLDGSWSLYRSRTLEVSEGDRVRALGRELKGAIKAKEQFTVAGLENGAVRLRSGDRELRLPTE
RAVKLTHDYVEGTGAGTSASRTVLAAVGPRGLNKQTLNALAQSGSDIRIYTPLTPEQAARKVESVSAVRL
ASDQVRQSTGEANLDTAIQASRERLMSDAEQAVNLAIPSAQQGQVYLSELTLLAEAVKSGQPLADVRAEI
ARQVDSGALIKLDSVAGAGNRVLVPRVAYEMEKTIIRHIAEGKEAVQPLMALTPASVLAGLTAGQREATR
TVLENTDRFMAIQGYAGVGKTTQFRAVMGALNTLSESVRPQVIGLGPTHRAVHEMREAGVDARTLASFLS
ETRLAIQAGETPDFRNVLFLTDESSMVGNRDMAELYQLVAAGGGRMVSSGDTAQLQAISTGLPFRLVQQR
SAIDTVVMQEIVRQTPALRPAIESIIAGQVEISLRQVDDVTPQQVPRQPGAWVPDNSVMEIRAPKKEQDQ
VQNIPVAGEQTLTPEQLALVRTDIIEAIRDDWMGRTPEAQQQTLVVAELNADRHAINEAIHTARHEKGDT
GAEERTFTVLEPLRVPDNALRAAETFAEYTGAVAMMNERYWTVAEVDTQDAVVTLRNADGESVLISPQQN
TAQDISLFTPRDLTISQGDRVRFTRSDTDRGYVANSLWEVAGFTDEGAIRFRQGDQEKIVDPQAMTEDRH
IDLAYALTVYGVQGASERFAIALTGTEGGRKRMASLESTYVTLSRAKEHVQVYTDDLAGWSADARHSNAG
QTAHDLLHQKSDHESDTGNRLLATASRLDKTALGRRVLAENGLEGETMARFIAAGKKYPSPYVALPAWTR
HGKAAGALLTEIRIEDDGMRVVLSDESRLRGGEDAQFAGLQASRNGQTLIADDAQTALRLAQENPESGVV
IRLHGEERLLNAARLTGGRITEPDEVERTVRSVAEAESAAKAEDPITLPPDEQQKLAEAQEKAAQELAEQ
ARQDLLPALPGTEGDKPDPLLSASDERRLRDGTERETRELDEAVREAVAEGREPRQQVRDQMQRTEREWV
VNVPEKEIELEKTLGGD

Protein domains

Predicted by InterproScan

• DNA relaxase TraI 2B/2B-like domain (TraI_2B) (644-723)

• TrwC relaxase (TrwC) (12-279)

• single-stranded DNA binding TraI N-terminal subdomain (ssDNA_TraI_N) (586-638)

• DNA helicase TraI (TraI_C) (1441-1558)

• AAA domain (AAA_30) (971-1155)

Protein structure

No available structure.

Reference

[1] Lu J et al. (2002) Analysis and characterization of the IncFV plasmid pED208 transfer region. Plasmid. 48(1):24-37. [PMID:12206753]
[2] Di Laurenzio L et al. (1991) Characterization of the oriT region of the IncFV plasmid pED208. Mol Microbiol. 5(7):1779-90. [PMID:1943709]