Detailed information of relaxase

Relaxase

• ID: 5
• GenBank: AAA99466
• Name: TraA_pIP501
• UniProt ID: Q52001
• Family: MOB_Q
• PDB ID: _
• Length: 654 a.a.
• Note: (1) The N-terminal domin (246 amino acids) of relaxase TraA_pIP501 show specific oriT binding [PMID:15554903]. (2) The relaxase TraA_pIP501 and its N-terminal domin (246 amino acids) behave as dimers in solution [PMID:15554903]. (3) The relaxase TraA_pIP501 negatively autoregulate the downstream 15 tra gene cluster [PMID:16514144].

Protein sequence

• Length: 654 a.a.
• Molecular weight: 76493.35 Da
• Isoelectric Point: 9.3594

>AAA99466.1 ORF1 (plasmid) [Plasmid pIP501]
MTIAKRENGKRSLIAMASYRSGEKLYSELYEKTNLYNHRTVKPEAFILKPDYVPNEFLDRQTLWNKMELA
EKSPNAQLCREVNVALPIELNNSDQRMLIEDFVKDNFVNEGMIADVAIHRDDENNPHAHIMLTMREVDSE
GNILNKSHRIPKLDENGNQIFNEKGQRVTVSIKTNDWGRKSLVSEIRKDWADKVNQYLKDRNIDQQITEK
SHAELGKKELPTIHEGFYSKKLEDKGVISELKRKNLEIQSYNDILAELDKLENQEKVLKQDQNFTLKFEK
TFSPLEKGELKNLSKELKLFINDENIDKRLGELKRWENSLIFNNKMEIQKQRLMLSKISSERDMLTKANE
ILDKQAERFFKKSYPSLNIDKFSNHEVRAMVNETIFRKQLLNKDQLAEVIYNERVVEKEESKKIFKEKPF
QTSRYLDSKIKQIEDSITKENNPERKEILSIKKEKLIGIKQGLIEYVQSEVERKFDKNVSIDSVIEGEML
LAKADYYKTTDFSKVEGVARFSSEEINSMLEQSKGFLTNIQTVKIPNDCQGVFFVQDSMKHIDELSPLAK
QNLKKVVNRNAYLPDSDKIELSKEIENTNKDQSQELDKDVPEKNEVTVKMFQFAKSINRLLSGNQLQKKR
NLDKLIKQTKAKKNQSLQRNIPLR

Protein domains

Predicted by InterproScan

• Nicking enzyme C-terminal middle helical domain (NES_C_h) (282-387)

• MobA/MobL family (MobA_MobL) (11-235)

Protein structure

• AlphaFold DB: Q52001

Reference

[1] Kurenbach B et al. (2006) The TraA relaxase autoregulates the putative type IV secretion-like system encoded by the broad-host-range Streptococcus agalactiae plasmid pIP501. Microbiology. 152(Pt 3):637-45. [PMID:16514144]
[2] Kurenbach B et al. (2003) Intergeneric transfer of the Enterococcus faecalis plasmid pIP501 to Escherichia coli and Streptomyces lividans and sequence analysis of its tra region. Plasmid. 50(1):86-93. [PMID:12826062]