Detailed information of relaxase

Relaxase

• ID: 270
• GenBank: AAL59675
• Name: TraI_SXT(MO10)
• UniProt ID: Q8KQY3
• Family: MOB_H
• PDB ID: _
• Length: 716 a.a.
• Note: The relaxase TraI_SXT(MO10) and the oriT_SXT(MO10) are key factors for SXT(MO10) replication and stability. After deletion the relaxase TraI_SXT(MO10) or the oriT_SXT(MO10), there are ~5-fold reduction of SXT(MO10) copy numbers [PMID:26061412].

Protein sequence

• Length: 716 a.a.
• Molecular weight: 79841.81 Da
• Isoelectric Point: 5.3504

>AAL59675.1 conjugative relaxase [Vibrio cholerae]
MFKNLFFQAKALPDLSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNLRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWLRKSNQSTHLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLATKSA
SNERYESLAPEVLIKDDKPIWLPMLHISEADLLFSSNVPSSVRLFSKSEWEATQKTQEEPQSHSSEHSDL
PEASSSIEHRKSDESPSTNSSEQENELRHASDVSNPQATENAPGDECEKPNDSSDADIANNANQHHAEAL
NLPESLAWLPEASSALVMVGEQVLIRYPDAVRHWCAPRKLLAELSRLDWLELDPANLTRKARTVTTNDGV
QEQGLLLKVSISKGLTALIDLPKQDAEPAAAIQNEEALQRPSRTETTNAQAKEPATRAERKQKPIAANTN
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDQC
QFDEGETVLFTAHAKR

Protein domains

Predicted by InterproScan

• Putative helicase (TraI_2) (39-349)

Protein structure

• AlphaFold DB: Q8KQY3

Reference

[1] Ceccarelli D et al. (2008) Identification of the origin of transfer (oriT) and a new gene required for mobilization of the SXT/R391 family of integrating conjugative elements. J Bacteriol. 190(15):5328-38. [PMID:18539733]