Detailed information of relaxase

Relaxase

• ID: 220
• GenBank: CAB12294
• Name: YdcR(NicK)_ICEBs1
• UniProt ID: P96635
• Family: MOB_T
• PDB ID: _
• Length: 352 a.a.
• Note: With the help of relaxase YdcR(NicK)_ICEBs1, a helicase processivity factor (HelP, formerly YdcP) can associate with the oriT_ICEBs1 in vivo [PMID:23326247].

Protein sequence

• Length: 352 a.a.
• Molecular weight: 40943.59 Da
• Isoelectric Point: 5.1091

>CAB12294.1 ICEBs1 mobile element: DNA relaxase [Bacillus subtilis subsp. subtilis str. 168]
MDELKQPPHANRGVVIVKEKNEAVESPLVSMVDYIRVSFKTHDVDRIIEEVLHLSKDFMTEKQSGFYGYV
GTYELDYIKVFYSAPDDNRGVLIEMSGQGCRQFESFLECRKKTWYDFFQDCMQQGGSFTRFDLAIDDKKT
YFSIPELLKKAQKGECISRFRKSDFNGSFDLSDGITGGTTIYFGSKKSEAYLCFYEKNYEQAEKYNIPLE
ELGDWNRYELRLKNERAQVAIDALLKTKDLTLIAMQIINNYVRFVDADENITREHWKTSLFWSDFIGDVG
RLPLYVKPQKDFYQKSRNWLRNSCAPTMKMVLEADEHLGKTDLSDMIAEAELADKHKKMLDVYMADVADM
VV

Protein domains

Predicted by InterproScan

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (31-119)

• Replication initiation factor (Rep_trans) (127-327)

Protein structure

• AlphaFold DB: P96635

Reference

[1] Lee CA et al. (2012) The Bacillus subtilis conjugative transposon ICEBs1 mobilizes plasmids lacking dedicated mobilization functions. J Bacteriol. 194(12):3165-72. [PMID:22505685]
[2] Lee CA et al. (2010) Autonomous plasmid-like replication of a conjugative transposon. Mol Microbiol. 75(2):268-79. [PMID:19943900]
[3] Lee CA et al. (2007) Identification of the origin of transfer (oriT) and DNA relaxase required for conjugation of the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 189(20):7254-61. [PMID:17693500]