Detailed information of relaxase

Relaxase

• ID: 215
• GenBank: AAB60013
• Name: Orf20_Tn916
• UniProt ID: Q47728
• Family: MOB_T
• PDB ID: _
• Length: 329 a.a.
• Note: Endonuclease

Protein sequence

• Length: 329 a.a.
• Molecular weight: 39099.72 Da
• Isoelectric Point: 7.0754

>AAB60013.1 ORF20 [Enterococcus faecalis]
MLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGC
RQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGE
LVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLLVY
DNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQVAP
TLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

Protein domains

Predicted by InterproScan

• Rolling Circle replication initiation protein N-terminal domain (Rol_Rep_N) (2-89)

• Replication initiation factor (Rep_trans) (97-301)

Protein structure

• AlphaFold DB: Q47728

Reference

[1] Rocco JM et al. (2006) The integrase of the conjugative transposon Tn916 directs strand- and sequence-specific cleavage of the origin of conjugal transfer, oriT, by the endonuclease Orf20. J Bacteriol. 188(6):2207-13. [PMID:16513750]