Detailed information of relaxase

Relaxase

• ID: 1069
• GenBank: WP_003693100
• Name: TraI_GGI
• UniProt ID: D6H7W1
• Family: MOB_H
• PDB ID: _
• Length: 850 a.a.
• Note: The MOB_H family relaxase TraI_GGI cleaves oriT_GGI in a site-specific and Mn²⁺-dependent manner [PMID:21255116, PMID:22016851].

Protein sequence

• Length: 850 a.a.
• Molecular weight: 94671.82 Da
• Isoelectric Point: 5.9293

>WP_003693100.1 MobH family relaxase [Neisseria gonorrhoeae]
MKTSLLTIASSLMLVSGSMLYLINTRKSTDSVSHNDEVAHINQNIVNDRFRILNAHELIQVLDLSPQISG
IKMNLGLSDENWSKDALPFLEKYIAFVQRLPASESHHHAGDGGLVRHTLDVAALALVASTSQSWPPNAKT
EEIAKKTAVWRYGIMCAALLHDVGKTVTGFQVELFDSAISLEKLLWLPDTGSMAESGKLYYRVEFPDAKS
AYSTHAEIAWTFFQALVPSHVRQWLATTDPNLMITLRNYLSGKKDGSPLEQLIKNADMTSVSRDLRSGSR
QRFSTAKRKPFIETIMETLKEMLSDRGVHFSIATTAGGDLFRKGDRIYLMSKNVPDYIRQYLRKNQHPAA
GSFPADNQRIFDTLFEYRAVIPPENDPHRAINHIEVEFTRMDGEKVRNIFSVLCFNAKTLYPDGDYPTEF
LGNLSEVSAKKEIPDVVVSEDKVSTQIQGAEDNPVTMTKDKMDFLSIQPVTAEQPPLPEKNQTETANSAF
DNSMNTAAPAENKSSESEKAKEPGYGTIDNLIENFNLIEADSEQTESTKSEAETAENAVNLVQENIRVEV
KTETVDKPRAEVKETETDKAPKTIKSGTKASRKKLAGLFADNRKPIGKTAEPDAQIKTTETVTHQIDSEA
VSLPESESAQNGMRELERLRLREPTSSPRPVQVMDDSEGLDSLASGVKNMAELEAIVSERNLKKQSETES
TEIVEDSVKAKHMEARDRGMQFLRWLADGLGDGSIAVNRSGATVHFIEQGMLLVTPAVFRDYAGGVFNKS
DTESLGPLTQKGFEALRLHARTKKSSLHRVLTTNGSNRRLFYCYLIPEHNIKHIIQPGSRPQNNTDIKLD
ESDLLVMEQK

Protein domains

Predicted by InterproScan

• Putative helicase (TraI_2) (60-372)

• Putative conjugal transfer nickase/helicase TraI C-term (TraI_2_C) (718-821)

Protein structure

• AlphaFold DB: D6H7W1

Reference

[1] Jan-Hendrik Heilers et al. (2019) DNA processing by the MOBH family relaxase TraI encoded within the gonococcal genetic island.. Nucleic acids research. 47(15):8136-8153. [PMID:31276596]