Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   400002
Name   oriT_Tn5070 experimental
Organism   Synthetic construct Streptomyces coelicolor
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   HQ259113 (3292..3391 [-], 100 nt)
oriT length   100 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RK2

  oriT sequence  


Download         Length: 100 nt

>oriT_Tn5070
AGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Fernández-Martínez LT et al. (2011) A transposon insertion single-gene knockout library and new ordered cosmid library for the model organism Streptomyces coelicolor A3(2). Antonie Van Leeuwenhoek. 99(3):515-22. [PMID:20945092]


Host bacterium


ID   2 Element type   
Element name   Synthetic construct Streptomyces coelicolor transposon Tn5070 GenBank   HQ259113
Element size   3981 bp Coordinate of oriT [Strand]   3292..3391 [-]
Host bacterium   Synthetic construct Streptomyces coelicolor

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -