Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300470 |
| Name | oriT_pAM120  |
| Organism | Cloning vector pAM120 |
| Sequence Completeness | intact |
| NCBI accession of oriT (coordinates [strand]) | U49939 (_) |
| oriT length | 216 nt |
| IRs (inverted repeats) | 61..76, 80..95 (ACTTAACCCCCCGTAT..ACAGGGGGGTACAAAT) 123..134, 139..150 (GAAAATCCTTTG..CAAGGGATTTAC) |
| Location of nic site | 135..136 |
| Conserved sequence flanking the nic site |
TGG|T |
| Note | oriT_Tn916 |
oriT sequence
Download Length: 216 nt
>oriT_pAM120
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGGCCTTTTTGATTGGAGGGATTT
AAGCGGAAGTCGCAGGTGTGGACTGATCTTGCTGGCTGGTGTGGCAATAGCCACGCCAGCACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATACGGCCTTTTTGATTGGAGGGATTT
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Gawron-Burke C et al. (1984) Regeneration of insertionally inactivated streptococcal DNA fragments after excision of transposon Tn916 in Escherichia coli: strategy for targeting and cloning of genes from gram-positive bacteria. J Bacteriol. 159(1):214-21. [PMID:6330031]
Host bacterium
| ID | 1455 | GenBank | U49939 |
| Plasmid name | Cloning vector pAM120 | Incompatibility group | - |
| Plasmid size | 23363 bp | Coordinate of oriT [Strand] | |
| Host baterium | Cloning vector pAM120 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |