Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300464
Name   oriT_pSB1 in_silico
Organism   Cloning vector pSB1 tetR tetA genes with Agrobacterium tumefaciens
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   AB027255 (26522..26633 [-], 112 nt)
oriT length   112 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4/RK2

  oriT sequence  


Download         Length: 112 nt

>oriT_pSB1
CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Komari T et al. (1996) Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers. Plant J. 10(1):165-74. [PMID:8758986]


Host bacterium


ID   677 GenBank   AB027255
Plasmid name   Cloning vector pSB1 tetR, tetA genes with Agrobacterium tumefaciens DNA insert Incompatibility group   ColRNAI
Plasmid size   36909 bp Coordinate of oriT [Strand]   26522..26633 [-]
Host baterium   Cloning vector pSB1 tetR tetA genes with Agrobacterium tumefaciens

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -