Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   300446
Name   oriT_pMo130 experimental
Organism   Burkholderia suicide vector pMo130
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   EU862243 (_)
oriT length   544 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site    		  
Note   oriT_RK2

  oriT sequence  


Download         Length: 544 nt

>oriT_pMo130
AGGAACGCAACCGCAGCCTCATCACGCCGGCGCTTCTTGGCCGCGCGGGATTCAACCCACTCGGCCAGCTCGTCGGTGTAGCTCTTTGGCATCGTCTCTCGCCTGTCCCCTCAGTTCAGTAATTTCCTGCATTTGCCTGTTTCCAGTCGGTAGATATTCCACAAAACAGCAGGGAAGCAGCGCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCAAGGTGTACTGCCTTCCAGACGAACGAAGAGCGATTGAGG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Hamad MA et al. (2009) An allelic exchange system for compliant genetic manipulation of the select agents Burkholderia pseudomallei and Burkholderia mallei. Gene. 430(1-2):123-31. [PMID:19010402]


Host bacterium

ID   659 GenBank   EU862243
Plasmid name   Burkholderia suicide vector pMo130 Incompatibility group   ColRNAI
Plasmid size   6127 bp Coordinate of oriT [Strand]   
Host baterium   Burkholderia suicide vector pMo130

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -