Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300445 |
| Name | oriT_pPSX  |
| Organism | Cloning vector pPSX |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | FJ422118 (12580..12981 [-], 402 nt) |
| oriT length | 402 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oirT_pR388 |
oriT sequence
Download Length: 402 nt
>oriT_pPSX
CTCATTTTCTGCATCATTGTAGCACCATCATAGCATTATAGTTGCATCATTGCTGCACGATAACCCAATGCGCATAGCGCATTGGGAGCAGTTTTGACCCTCTTTAGGGTCACGCTGGCGTTATCGCATGGTTTCCGTCCTTAAAAGCCGGGTTGGTATCCTTAAATCTGGGCTATAGACAATACGCACCTTTCGGTGCGCGTAAGTCATTGATTTACAATGACTTCCACGCCAAGGACGAAAACCTGTGTAGTGTTATGCCACTACAATATTGCCGCAACCCCTTGTAAATGCTAGGATTGAACCACTACAGTAACACTACGGGAGAGGACATGGCACTAGGCGACCCCATCCAAGTACGGCTAAGCCCGGAAAAGCAGGCACTTTTGGAGGACGAGGCGG
CTCATTTTCTGCATCATTGTAGCACCATCATAGCATTATAGTTGCATCATTGCTGCACGATAACCCAATGCGCATAGCGCATTGGGAGCAGTTTTGACCCTCTTTAGGGTCACGCTGGCGTTATCGCATGGTTTCCGTCCTTAAAAGCCGGGTTGGTATCCTTAAATCTGGGCTATAGACAATACGCACCTTTCGGTGCGCGTAAGTCATTGATTTACAATGACTTCCACGCCAAGGACGAAAACCTGTGTAGTGTTATGCCACTACAATATTGCCGCAACCCCTTGTAAATGCTAGGATTGAACCACTACAGTAACACTACGGGAGAGGACATGGCACTAGGCGACCCCATCCAAGTACGGCTAAGCCCGGAAAAGCAGGCACTTTTGGAGGACGAGGCGG
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Sarovich DS et al. (2007) pPSX: a novel vector for the cloning and heterologous expression of antitumor antibiotic gene clusters. Plasmid. 57(3):306-13. [PMID:17218012]
Host bacterium
| ID | 658 | GenBank | FJ422118 |
| Plasmid name | Cloning vector pPSX | Incompatibility group | IncW |
| Plasmid size | 14757 bp | Coordinate of oriT [Strand] | 12580..12981 [-] |
| Host baterium | Cloning vector pPSX |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |