Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   300412
Name   oriT_pFLPe2 experimental
Organism   Site-specific excision vector pFLPe2
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   EU215437 (_)
oriT length   387 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site    		  
Note   oriT_RK2

  oriT sequence  


Download         Length: 387 nt

>oriT_pFLPe2
GGGGATAACGCAGGAAAGAACATGGGGATTCCTTAAGGTATACTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGATAAGCTAGCTTATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCAT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Choi KH et al. (2008) Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl Environ Microbiol. 74(4):1064-75. [PMID:18156318]


Host bacterium

ID   625 GenBank   EU215437
Plasmid name   Site-specific excision vector pFLPe2 Incompatibility group   ColRNAI
Plasmid size   6611 bp Coordinate of oriT [Strand]   
Host baterium   Site-specific excision vector pFLPe2

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -