Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300390 |
| Name | oriT_pLC-ccdB  |
| Organism | MISSA donor vector pLC-ccdB |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | GU574773 (5604..5925 [-], 322 nt) |
| oriT length | 322 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 322 nt
>oriT_pLC-ccdB
TTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCA
TTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCA
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Chen QJ et al. (2010) MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation. Plant Physiol. 153(1):41-51. [PMID:20200068]
Host bacterium
| ID | 603 | GenBank | GU574773 |
| Plasmid name | MISSA donor vector pLC-ccdB | Incompatibility group | ColRNAI |
| Plasmid size | 5931 bp | Coordinate of oriT [Strand] | 5604..5925 [-] |
| Host baterium | MISSA donor vector pLC-ccdB |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |