Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300385
Name   oriT_BIBAC-LTR experimental
Organism   MISSA recipient vector BIBAC-LTR
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   GU574780 (14932..15981 [-], 1050 nt)
oriT length   1050 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4

  oriT sequence  


Download         Length: 1050 nt

>oriT_BIBAC-LTR
GATCGTGGCATCACCGAACCGCGCCGTGCGCGGGTCGTCGGTGAGCCAGAGTTTCAGCAGGCCGCCCAGGCGGCCCAGGTCGCCATTGATGCGGGCCAGCTCGCGGACGTGCTCATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAGCTACACCGACGAGCTGGCCGAGTGGGTTGAATCCCGCGCGGCCAAGAAGCGCCGGCGTGATGAGGCTGCGGTTGCGTTCCTGGCGGTGAGGGCGGATGTCGAGGCGGCGTTAGCGTCCGGCTATGCGCTCGTCACCATTTGGGAGCACATGCGGGAAACGGGGAAGGTCAAGTTCTCCTACGAGACGTTCCGCTCGCACGCCAGGCGGCACATCAAGGCCAAGCCCGCCGATGTGCCCGCACCGCAGGCCAAGGCTGCGGAACCCGCGCCGGCACCCAAGACGCCGGAGCCACGGCGGCCGAAGCAGGGGGGCAAGGCTGAAAAGCCGGCCCCCGCTGCGGCCCCGACCGGCTTCACCTTCAACCCAACACCGGACAAAAAGGGATC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Chen QJ et al. (2010) MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation. Plant Physiol. 153(1):41-51. [PMID:20200068]


Host bacterium


ID   598 GenBank   GU574780
Plasmid name   MISSA recipient vector BIBAC-LTR Incompatibility group   IncFIA
Plasmid size   16019 bp Coordinate of oriT [Strand]   14932..15981 [-]
Host baterium   MISSA recipient vector BIBAC-LTR

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -