Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300382 |
| Name | oriT_pUC18T-mini-Tn7T-LAC-Gm  |
| Organism | Expression vector pUC18T-mini-Tn7T-LAC-Gm |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | EU240902 (_) |
| oriT length | 268 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 268 nt
>oriT_pUC18T-mini-Tn7T-LAC-Gm
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCC
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Choi KH et al. (2008) Genetic tools for select-agent-compliant manipulation of Burkholderia pseudomallei. Appl Environ Microbiol. 74(4):1064-75. [PMID:18156318]
Host bacterium
| ID | 595 | GenBank | EU240902 |
| Plasmid name | Expression vector pUC18T-mini-Tn7T-LAC-Gm | Incompatibility group | ColRNAI |
| Plasmid size | 6047 bp | Coordinate of oriT [Strand] | |
| Host baterium | Expression vector pUC18T-mini-Tn7T-LAC-Gm |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |