Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300380
Name   oriT_miniTn7-gat-gfp experimental
Organism   Site-specific integration vector miniTn7-gat-gfp
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   HM150705 (_)
oriT length   230 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4

  oriT sequence  


Download         Length: 230 nt

>oriT_miniTn7-gat-gfp
TCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Norris MH et al. (2010) Stable, site-specific fluorescent tagging constructs optimized for Burkholderia species. Appl Environ Microbiol. 76(22):7635-40. [PMID:20851961]


Host bacterium


ID   593 GenBank   HM150705
Plasmid name   Site-specific integration vector miniTn7-gat-gfp Incompatibility group   -
Plasmid size   3325 bp Coordinate of oriT [Strand]   
Host baterium   Site-specific integration vector miniTn7-gat-gfp

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -