Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300368
Name   oriT_pVec8-GFP in_silico
Organism   Binary vector pVec8-GFP
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   FJ949107 (_)
oriT length   249 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   _

  oriT sequence  


Download         Length: 249 nt

>oriT_pVec8-GFP
GGATCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGG

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Murray F et al. (2004) Comparison of Agrobacterium-mediated transformation of four barley cultivars using the GFP and GUS reporter genes. Plant Cell Rep. 22(6):397-402. [PMID:14530864]


Host bacterium


ID   581 GenBank   FJ949107
Plasmid name   Binary vector pVec8-GFP Incompatibility group   IncP1
Plasmid size   14559 bp Coordinate of oriT [Strand]   
Host baterium   Binary vector pVec8-GFP

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -