Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300358 |
| Name | oriT_pUC18T-mini-Tn7T-Gm-DsRedExpress  |
| Organism | Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Gm-DsRedExpress |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | DQ493880 (_) |
| oriT length | 269 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 269 nt
>oriT_pUC18T-mini-Tn7T-Gm-DsRedExpress
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Choi KH et al. (2006) Mini-Tn7 insertion in bacteria with single attTn7 sites: example Pseudomonas aeruginosa. Nat Protoc. 1(1):153-61. [PMID:17406227]
Host bacterium
| ID | 571 | GenBank | DQ493880 |
| Plasmid name | Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Gm-DsRedExpress | Incompatibility group | ColRNAI |
| Plasmid size | 5725 bp | Coordinate of oriT [Strand] | |
| Host baterium | Mini-Tn7 delivery vector pUC18T-mini-Tn7T-Gm-DsRedExpress |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |