Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   300342
Name   oriT_pUC18R6KT-mini-Tn7T experimental
Organism   Mini-Tn7 delivery vector pUC18R6KT-mini-Tn7T
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   AY712953 (_)
oriT length   262 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site    		  
Note   oriT_RP4

  oriT sequence  


Download         Length: 262 nt

>oriT_pUC18R6KT-mini-Tn7T
CCTTAAGGTATACTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGATAAGCT

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Choi KH et al. (2005) A Tn7-based broad-range bacterial cloning and expression system. Nat Methods. 2(6):443-8. [PMID:15908923]


Host bacterium

ID   555 GenBank   AY712953
Plasmid name   Mini-Tn7 delivery vector pUC18R6KT-mini-Tn7T Incompatibility group   -
Plasmid size   3485 bp Coordinate of oriT [Strand]   
Host baterium   Mini-Tn7 delivery vector pUC18R6KT-mini-Tn7T

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -