Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300243 |
| Name | oriT_pTJ1  |
| Organism | Cloning vector pTJ1 |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | JX559783 (_) |
| oriT length | 269 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 269 nt
>oriT_pTJ1
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Damron FH et al. (2013) Construction of a broad-host-range Tn7-based vector for single-copy PBAD-controlled gene expression in gram-negative bacteria. Appl Environ Microbiol. 79(2):718-21. [PMID:23124231]
Host bacterium
| ID | 456 | GenBank | JX559783 |
| Plasmid name | Cloning vector pTJ1 | Incompatibility group | ColRNAI |
| Plasmid size | 5667 bp | Coordinate of oriT [Strand] | |
| Host baterium | Cloning vector pTJ1 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |