Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   300232
Name   oriT_pKFRT/FLP experimental
Organism   Suicide vector for unmarked mutagenesis pKFRT/FLP
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   AB773262 (_)
oriT length   549 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site    		  
Note   oriT_RP4

  oriT sequence  


Download         Length: 549 nt

>oriT_pKFRT/FLP
ATAGTCCACGACGCCCGTGATTTTGTAGCCCTGGCCGACGGCCAGCAGGTAGGCCGACAGGCTCATGCCGGCCGCCGCCGCCTTTTCCTCAATCGCTCTTCGTTCGTCTGGAAGGCAGTACACCTTGATAGGTGGGCTGCCCTTCCTGGTTGGCTTGGTTTCATCAGCCATCCGCTTGCCCTCATCTGTTACGCCGGCGGTAGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCCGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAAGCGCTGCTTCCCTGCTGTTTTGTGGAATATCTACCGACTGGAAACAGGCAAATGCAGGAAATTACTGAACTGAGGGGACAGGCGAGAGACGATGCCAAAGAG

Visualization of oriT structure (The oriT was characterized experimentally)


  Reference


[1] Ishikawa M et al. (2013) A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination. BMC Microbiol. 0.601388889. [PMID:23594401]


Host bacterium

ID   445 GenBank   AB773262
Plasmid name   Suicide vector for unmarked mutagenesis pKFRT/FLP Incompatibility group   ColRNAI
Plasmid size   6419 bp Coordinate of oriT [Strand]   
Host baterium   Suicide vector for unmarked mutagenesis pKFRT/FLP

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -