Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300215 |
| Name | oriT_pUC18T-mini-Tn7T-lux-Tp  |
| Organism | Reporter vector pUC18T-mini-Tn7T-lux-Tp |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | KC848883 (_) |
| oriT length | 269 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 269 nt
>oriT_pUC18T-mini-Tn7T-lux-Tp
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
AGCTTATCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCTGACGCCGTTGGATACACCAAGGAAAGTCTACACGAACCCTTTGGCAAAATCCTGTATATCGTGCGAAAAAGGATGGATATACCGAAAAAATCGCTATAATGACCCCGAAGCAGGGTTATGCAGCGGAAAGTATACCTTAAGGAATCCCC
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Damron FH et al. (2013) Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis. Appl Environ Microbiol. 79(13):4149-53. [PMID:23584769]
Host bacterium
| ID | 428 | GenBank | KC848883 |
| Plasmid name | Reporter vector pUC18T-mini-Tn7T-lux-Tp | Incompatibility group | ColRNAI |
| Plasmid size | 11738 bp | Coordinate of oriT [Strand] | |
| Host baterium | Reporter vector pUC18T-mini-Tn7T-lux-Tp |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |