Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300190
Name   oriT_pV3SDlacZ experimental
Organism   Cloning vector pV3SDlacZ
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   KJ569569 (6134..6359 [-], 226 nt)
oriT length   226 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4

  oriT sequence  


Download         Length: 226 nt

>oriT_pV3SDlacZ
ATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Hertel R et al. (2015) Conjugative reporter system for the use in Bacillus licheniformis and closely related Bacilli. Lett Appl Microbiol. 60(2):162-7. [PMID:25363901]


Host bacterium


ID   403 GenBank   KJ569569
Plasmid name   Cloning vector pV3SDlacZ Incompatibility group   ColRNAI
Plasmid size   7586 bp Coordinate of oriT [Strand]   6134..6359 [-]
Host baterium   Cloning vector pV3SDlacZ

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -