Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 300187 |
Name | oriT_pSEVA12DKR-E |
Organism | Synthetic construct clone pSEVA12DKR-E |
Sequence Completeness | |
NCBI accession of oriT (coordinates [strand]) | KJ686605 (_) |
oriT length | 236 nt |
IRs (inverted repeats) | |
Location of nic site | |
Conserved sequence flanking the nic site |
|
Note | oriT_RP4 |
oriT sequence
Download Length: 236 nt
>oriT_pSEVA12DKR-E
GCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTA
GCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTA
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure fileReference
[1] Wright O et al. (2015) GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 4(3):307-16. [PMID:24847673]
Host bacterium
ID | 400 | GenBank | KJ686605 |
Plasmid name | Synthetic construct clone pSEVA12DKR-E | Incompatibility group | IncX2 |
Plasmid size | 8156 bp | Coordinate of oriT [Strand] | |
Host baterium | Synthetic construct clone pSEVA12DKR-E |
Cargo genes
Drug resistance gene | - |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | - |