Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300186 |
| Name | oriT_pSEVA12DKR-J  |
| Organism | Synthetic construct clone pSEVA12DKR-J |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | KJ686606 (_) |
| oriT length | 236 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 236 nt
>oriT_pSEVA12DKR-J
GCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTA
GCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGTA
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Wright O et al. (2015) GeneGuard: a Modular Plasmid System Designed for Biosafety. ACS Synth Biol. 4(3):307-16. [PMID:24847673]
Host bacterium
| ID | 399 | GenBank | KJ686606 |
| Plasmid name | Synthetic construct clone pSEVA12DKR-J | Incompatibility group | IncX2 |
| Plasmid size | 8156 bp | Coordinate of oriT [Strand] | |
| Host baterium | Synthetic construct clone pSEVA12DKR-J |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |