Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300171 |
| Name | oriT_pA1.GFP.A2  |
| Organism | AAVS1 targeting vector pA1.GFP.A2 |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | GQ380658 (_) |
| oriT length | 296 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | _ |
oriT sequence
Download Length: 296 nt
>oriT_pA1.GFP.A2
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAACGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACACTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGT
CGCACCGCTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAACGGTGTCGGCGCGTTGTTGTAGCCGCGCCGACACCGCTTTTTTAAATATCATAAAGAGAGTAAGAGAAACTAATTTTTCATAACACTCTATTTATAAAGAAAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGT
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] van Nierop GP et al. (2009) Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease. Nucleic Acids Res. 37(17):5725-36. [PMID:19651880]
Host bacterium
| ID | 384 | GenBank | GQ380658 |
| Plasmid name | AAVS1 targeting vector pA1.GFP.A2 | Incompatibility group | - |
| Plasmid size | 12653 bp | Coordinate of oriT [Strand] | |
| Host baterium | AAVS1 targeting vector pA1.GFP.A2 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |