Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300163
Name   oriT_pRK415 experimental
Organism   Broad host range expression vector pRK415
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   EF437940 (_)
oriT length   110 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RK2

  oriT sequence  


Download         Length: 110 nt

>oriT_pRK415
CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Hülter N et al. (2008) Double illegitimate recombination events integrate DNA segments through two different mechanisms during natural transformation of Acinetobacter baylyi. Mol Microbiol. 67(5):984-95. [PMID:18194157]


Host bacterium


ID   376 GenBank   EF437940
Plasmid name   Broad host range expression vector pRK415 Incompatibility group   IncP1
Plasmid size   10690 bp Coordinate of oriT [Strand]   
Host baterium   Broad host range expression vector pRK415

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -