Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300155
Name   oriT_pJC24 experimental
Organism   Cloning vector pJC24
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   KC442291 (_)
oriT length   110 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RK2

  oriT sequence  


Download         Length: 110 nt

>oriT_pJC24
CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCC

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Cheng J et al. (2014) Versatile broad-host-range cosmids for construction of high quality metagenomic libraries. J Microbiol Methods. 99:27-34. [PMID:24495694]


Host bacterium


ID   368 GenBank   KC442291
Plasmid name   Cloning vector pJC24 Incompatibility group   IncP1
Plasmid size   13804 bp Coordinate of oriT [Strand]   
Host baterium   Cloning vector pJC24

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -