Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300110
Name   oriT_pKS800 experimental
Organism   Cloning vector pKS800
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   AB521037 (_)
oriT length   112 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RK2

  oriT sequence  


Download         Length: 112 nt

>oriT_pKS800
GCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Hattori Y et al. (2002) Ordered cosmid library of the Mesorhizobium loti MAFF303099 genome for systematic gene disruption and complementation analysis. Plant Cell Physiol. 43(12):1542-57. [PMID:12514252]


Host bacterium


ID   323 GenBank   AB521037
Plasmid name   Cloning vector pKS800 Incompatibility group   IncP1
Plasmid size   20392 bp Coordinate of oriT [Strand]   
Host baterium   Cloning vector pKS800

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -