Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300078
Name   oriT_JN100 experimental
Organism   Expression vector JN100
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   DQ309424 (_)
oriT length   119 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RK2

  oriT sequence  


Download         Length: 119 nt

>oriT_JN100
ATATGCCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGGCA

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Nikodinovic J et al. (2006) A second generation snp-derived Escherichia coli-Streptomyces shuttle expression vector that is generally transferable by conjugation. Plasmid. 56(3):223-7. [PMID:16806469]


Host bacterium


ID   291 GenBank   DQ309424
Plasmid name   Expression vector JN100 Incompatibility group   ColRNAI
Plasmid size   6460 bp Coordinate of oriT [Strand]   
Host baterium   Expression vector JN100

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -