Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300050
Name   oriT_pSW4426T experimental
Organism   Cloning vector pSW4426T
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   DQ995482 (35..291 [-], 257 nt)
oriT length   257 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4

  oriT sequence  


Download         Length: 257 nt

>oriT_pSW4426T
GGTACCAGCGCTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Le Roux F et al. (2007) Construction of a Vibrio splendidus mutant lacking the metalloprotease gene vsm by use of a novel counterselectable suicide vector. Appl Environ Microbiol. 73(3):777-84. [PMID:17122399]


Host bacterium


ID   263 GenBank   DQ995482
Plasmid name   Cloning vector pSW4426T Incompatibility group   -
Plasmid size   4424 bp Coordinate of oriT [Strand]   35..291 [-]
Host baterium   Cloning vector pSW4426T

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -