Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 300047 |
| Name | oriT_pMK2030  |
| Organism | Reporter vector pMK2030 |
| Sequence Completeness | |
| NCBI accession of oriT (coordinates [strand]) | EU825945 (_) |
| oriT length | 336 nt |
| IRs (inverted repeats) | |
| Location of nic site | |
| Conserved sequence flanking the nic site |
|
| Note | oriT_RP4 |
oriT sequence
Download Length: 336 nt
>oriT_pMK2030
AATTCCGTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCACGGAATT
AATTCCGTTTTCCGCTGCATAACCCTGCTTCGGGGTCATTATAGCGATTTTTTCGGTATATCCATCCTTTTTCGCACGATATACAGGATTTTGCCAAAGGGTTCGTGTAGACTTTCCTTGGTGTATCCAACGGCGTCAGCCGGGCAGGATAGGTGAAGTAGGCCCACCCGCGAGCGGGTGTTCCTTCTTCACTGTCCCTTATTCGCACCTGGCGGTGCTCAACGGGAATCCTGCTCTGCGAGGCTGGCCGGCTACCGCCGGCGTAACAGATGAGGGCAAGCGGATGGCTGATGAAACCAAGCCAACCAGGAAGGGCAGCCCACCTATCACGGAATT
Visualization of oriT structure (The oriT was characterized experimentally)
oriT secondary structure
Predicted by RNAfold.
Download structure file
Reference
[1] Humann JL et al. (2008) Construction and expression of sugar kinase transcriptional gene fusions by using the Sinorhizobium meliloti ORFeome. Appl Environ Microbiol. 74(21):6756-65. [PMID:18791020]
Host bacterium
| ID | 260 | GenBank | EU825945 |
| Plasmid name | Reporter vector pMK2030 | Incompatibility group | - |
| Plasmid size | 6926 bp | Coordinate of oriT [Strand] | |
| Host baterium | Reporter vector pMK2030 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |