Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   300030
Name   oriT_pSET152 experimental
Organism   Cloning vector PSET152
Sequence Completeness      
NCBI accession of oriT (coordinates [strand])   AJ414670 (_)
oriT length   112 nt
IRs (inverted repeats)    
Location of nic site      
Conserved sequence flanking the
  nic site  
 
 
Note   oriT_RP4

  oriT sequence  


Download         Length: 112 nt

>oriT_pSET152
CCGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG

Visualization of oriT structure (The oriT was characterized experimentally)

  oriT secondary structure

Predicted by RNAfold.

Download structure file

  Reference


[1] Sun Y et al. (2002) Streptomyces nanchangensis', a producer of the insecticidal polyether antibiotic nanchangmycin and the antiparasitic macrolide meilingmycin, contains multiple polyketide gene clusters. Microbiology. 148(Pt 2):361-71. [PMID:11832500]


Host bacterium


ID   243 GenBank   AJ414670
Plasmid name   Cloning vector pSET152 Incompatibility group   ColRNAI
Plasmid size   5715 bp Coordinate of oriT [Strand]   
Host baterium   Cloning vector PSET152

Cargo genes


Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -