Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200561
Name   oriT_IME_RhoA2-183_NS_2 in_silico
Organism   Roseburia hominis A2-183
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   NC_015977 (136..170 [+], 35 nt)
oriT length   35 nt
IRs (inverted repeats)      5..11, 15..21  (ACTTTGC..GCAAAGT)
Location of nic site      29..30
Conserved sequence flanking the
  nic site    		  GTGTGTTATA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 35 nt

>oriT_IME_RhoA2-183_NS_2
CACGACTTTGCGGAGCAAAGTGTAGTGTGTTATAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14590 GenBank   WP_002575862
Name   mobV_RHOM_RS10770_IME_RhoA2-183_NS_2 insolico UniProt ID   R0CNK4
Length   314 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 314 a.a.        Molecular weight: 36383.80 Da        Isoelectric Point: 10.3227

>WP_002575862.1 MULTISPECIES: MobV family relaxase [Bacteria]
MAQHAILRFEKHKGHPAGPLEAHHERKKEQYASNPDIDTSRSKYNFHIVKPDGRYYHFIQSRIEQARCRT
RKDSTRFVDTLITASPEFFKGKSPKEIAAYFQRAADFLIDRVGRENIVSAMVHMDEKTPHLHLVFVPLTK
DNRLCAKEIIGNRANLTKWQDDFHACMVEQYPDLERGESASKTGRKHIPTRLFKQAVNLSKQARAIEAVL
SGITPLNAGKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEVRAKASEKGKMNDTMERAK
LKSELDDMRRLVDRIPPEILAELKRQQRQHGKER

  Protein domains


Predicted by InterproScan.

Plasmid recombination enzyme (Mob_Pre)

(1-194)


  Protein structure


Source ID Structure
AlphaFold DB R0CNK4


Host bacterium

ID   500 Element type   IME (Integrative mobilizable element)
Element name   IME_RhoA2-183_NS_2 GenBank   NC_015977
Element size   3592125 bp Coordinate of oriT [Strand]   136..170 [+]
Host bacterium   Roseburia hominis A2-183 Coordinate of element   2395810..2403435

Cargo genes

Drug resistance gene   -
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA21