Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200523 |
| Name | oriT_ICEAspMex1  |
| Organism | Alteromonas sp. Mex14 |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | CP018023 (3194..3492 [+], 299 nt) |
| oriT length | 299 nt |
| IRs (inverted repeats) | 45..50, 52..57 (CAAACG..CGTTTG) 7..12, 26..31 (TTTGGC..GCCAAA) |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 299 nt
>oriT_ICEAspMex1
GCTCTGTTTGGCGGCTTGTGACCTAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
GCTCTGTTTGGCGGCTTGTGACCTAGCCAAAAAAATCGAGACGCCAAACGTCGTTTGCATTCTGGCCTGAACTTGCCAAACGGTTTGTATCTTCATGGCGATACGTCTTTTTAGGTGTTTTTAAGTGAAATCGGCCTGTATCCCTTGTCAGGTATGGGATTGCGCGAGTTGATTTATATCGAGACGCCAAACAGTGATTGTTACGGCAGTTTTACGTTTGGCGTTTCGATCCAAAAGCCAAACGGATAGTGGTTTTGGCTTTTGGGGTTAATTGGATGGGGAAATTGGTTTGGTAGAAA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14581 | GenBank | APD85582 |
| Name | TraI_2_BM527_05475_ICEAspMex1 |
UniProt ID | _ |
| Length | 716 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 716 a.a. Molecular weight: 79506.48 Da Isoelectric Point: 5.0396
>APD85582.1 phosphohydrolase [Alteromonas sp. Mex14]
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSINLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDGKPIWLPMLHISEADLLFSSNVPIGVTLFSKPEWEATQQTQAEPQSRSSEQPGL
SEASSSIDHSSSAESPSTKSSEQDDELRHASDVSNPQATENAPGDECEKPNNSYDGAISNNVNQYDTEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTAQIDLSKQDAESAAAIQNEEASPRPSLTETTNAQAKEPATRAERKQKPIAPNAQ
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDEC
QFDEGETVLFTAHAKR
MFKNLFFQTKALPELSSQLDAEIPRYPPFLKGLPAASPEDLQSTQDELIAKLRQVLGFNQRDFQRLIQPC
IDHLAAYVHLLPASEHHHHSGAGGLLRHSLEVAFWAAQAAEGIIFVASGTPVEKKELEPRWRVAAALGGL
FHDIGKPVSDLSITDEDGRYQWNPFLETLSQWTTNNSIERYFIRWRDGRCKRHEQFSILVLNRVMTPELL
AWLTQPGPEILQAMLEAIGNTDPEHVLSKLVIEADQTSVQRDLKAQRISVDDNALGVPVERYLLDAMRRL
LASSQWLVNQRDARVWVRKSNQSINLYLVWKSAAKDIIELLAKDKIPGIPRDPDTLADILIERGLAIKSA
SNERYESLAPEVLIKDGKPIWLPMLHISEADLLFSSNVPIGVTLFSKPEWEATQQTQAEPQSRSSEQPGL
SEASSSIDHSSSAESPSTKSSEQDDELRHASDVSNPQATENAPGDECEKPNNSYDGAISNNVNQYDTEAL
NLPESLAWLPEASSALVMVGEQILIRYPDAVRHWCAPRKLLAELSRLDWLELDPANPTRKARTVTTNDGV
QEQGLLLKVSISKGLTAQIDLSKQDAESAAAIQNEEASPRPSLTETTNAQAKEPATRAERKQKPIAPNAQ
SSTDPKHAQRQQMVNFVKDLPILLTDGDYPDVDHSADGIRVTIQTLRQVANAHGIPAGQLLRGISASDEC
QFDEGETVLFTAHAKR
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 17202 | GenBank | APD85583 |
| Name | t4cp2_BM527_05480_ICEAspMex1 |
UniProt ID | _ |
| Length | 606 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 606 a.a. Molecular weight: 67170.96 Da Isoelectric Point: 6.3833
>APD85583.1 conjugal transfer protein [Alteromonas sp. Mex14]
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMALSHLQKVMEKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIVAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FSARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPNTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
MKENAYEMPWRTNYEAMAAAGWLVGATGAIAAEMLTELPPEPFWWMTGISSGMALYRLPEAYRLYKLQKG
LKGKPLAFMALSHLQKVMEKHPDELWLGYGFEWDQRHAQRAYEILKRDKQTLLNQGHGKQMGSTWIHGVE
PKEEDVYQPVGHTEGHTLIVGTTGAGKTRCFDAMITQAILRNEAVIIIDPKGDKELKDNAQRACIVAGSP
ERFVYFHPGFPEHSVRLNPLRNFNRGTEIASRIAALIPSETGADPFKAFGQMALNNIVQGLLLTSQRPDL
KTLRRFLEGGPEGLVVKAVTAWGEQVYPNFSVEIKRFTEKANTLAKQAMAMLLFYYERIQPVAANTDLEG
LLSMFEHDRTHYSKMVASLMPVLNMLTSSELGPLLSPIANDVDDSRLITDSGRIINNAQVAYIGLDSLTD
AMVGSAIGSLLLSDLTAVAGDRYNYGVENRPVNIFIDEAAEVVNDPFIQLLNKGRGAKMRCVIATQTFAD
FSARTGSEAKARQVLGNINNLIALRVMDAETQQYITDNLPNTRLQYIMQTQGMSSNSDSPALFTGNHGER
LMEEEGDMFPPQLLGQLPNLEYIAKLSGGRVIKGRIPILTSSTQAV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
| ID | 17203 | GenBank | APD85598 |
| Name | t4cp2_BM527_05565_ICEAspMex1 |
UniProt ID | _ |
| Length | 799 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 799 a.a. Molecular weight: 90143.70 Da Isoelectric Point: 5.8187
>APD85598.1 type-IV secretion system protein TraC [Alteromonas sp. Mex14]
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYQVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
MKASLYSGQRASELLPVLAYSDDEQLFFMEDQSVGFGFLCDPLPGGDESVADRVNVLLNNDWPKDTLLQF
GLYASPDIQTDLQRMMGLRHRQSDPLLRASIRKRADFLDGGTVQPIEESTQTQVRNFQLIVTCKLPLESP
IPTERELSRASALRASFSQALATVGFRVTEMTDRNWLAALSAQLNWGKDASWRNPSPIRSEADKPLREQV
LDYDRAIKVDSQGLMLGDYQVKTLSFKRLPERIWFGHAASFAGDMMTGSRGLRGSFLLNVTIHFPSAEAM
RSRLETKRQWAVNQAYGPMLKFVPVLAAKKKGFDVLFEALQEGDRPIRANMTLTLFSPTEEASISSVSNA
RTYFKELGFELMEDKYFCLPIFLNALPFGADRQAMNDLFRFKTMATRHVIPLLPLFADWKGTGTPVINFV
SRNGQIMSVSLYDSGSNYNCCIAAQSGSGKSFLVNEIISSYLSEGGQCWVIDVGRSYEKLCEVYDGEFLQ
FGRDSGICLNPFEIVEDYDEEADVLVGLLAAMAAPTQSLTDFQMANLKRQTRELWEKKGRAMLVDDVAEA
LKNHEDRRVQDVGEQLYPFTTQGEYGRFFNGHNNIRFKNRFTVLELEELKGRKHLQQVVLLQLIYQIQQE
MYLGERDRRKIVFIDEAWDLLTQGDVGKFIETGYRRFRKYGGSAVTVTQSVNDLYDSPTGKAIAENSANM
YLLGQKAETINALKKEGRLPLGEGGYEYLKTVHTVTGVYSEIFFITEMGTGIGRLIVDPFHKLLYSSRAE
DVNAIKQLTRKGLSVADAISQLLKERGYE
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 1277099..1304384
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BM527_05470 | 1273562..1274743 | + | 1182 | APD85581 | hypothetical protein | - |
| BM527_05475 | 1274900..1277050 | + | 2151 | APD85582 | phosphohydrolase | - |
| BM527_05480 | 1277099..1278919 | + | 1821 | APD85583 | conjugal transfer protein | virb4 |
| BM527_05485 | 1278929..1279489 | + | 561 | APD85584 | conjugative transfer protein | - |
| BM527_05490 | 1279476..1280111 | + | 636 | APD85585 | conjugal transfer protein | tfc7 |
| BM527_05495 | 1280138..1280725 | - | 588 | APD85586 | plasmid-related protein | - |
| BM527_05500 | 1281014..1281295 | + | 282 | APD85587 | type IV conjugative transfer system protein TraL | traL |
| BM527_05505 | 1281292..1281918 | + | 627 | APD85588 | pilus assembly protein | traE |
| BM527_05510 | 1281902..1282798 | + | 897 | APD88100 | hypothetical protein | traK |
| BM527_05515 | 1282801..1284090 | + | 1290 | APD85589 | conjugal transfer protein TraB | traB |
| BM527_05520 | 1284087..1284737 | + | 651 | APD85590 | conjugal transfer protein TraV | traV |
| BM527_05525 | 1284734..1285120 | + | 387 | APD85591 | hypothetical protein | - |
| BM527_05530 | 1285341..1286120 | + | 780 | APD88101 | hypothetical protein | - |
| BM527_05535 | 1286125..1287063 | + | 939 | APD85592 | hypothetical protein | - |
| BM527_05540 | 1287642..1290371 | + | 2730 | APD85593 | heavy metal resistance protein CzcA | - |
| BM527_05545 | 1290368..1291477 | + | 1110 | APD85594 | hypothetical protein | - |
| BM527_05550 | 1291490..1293022 | + | 1533 | APD85595 | hypothetical protein | - |
| BM527_05555 | 1293025..1294470 | + | 1446 | APD85596 | AAA family ATPase | - |
| BM527_05560 | 1294615..1295307 | + | 693 | APD85597 | disulfide isomerase | trbB |
| BM527_05565 | 1295307..1297706 | + | 2400 | APD85598 | type-IV secretion system protein TraC | virb4 |
| BM527_05570 | 1297699..1298046 | + | 348 | APD85599 | plasmid-related protein | - |
| BM527_05575 | 1298123..1298542 | + | 420 | APD88102 | S26 family signal peptidase | - |
| BM527_05580 | 1298553..1299677 | + | 1125 | APD85600 | pilus assembly protein | traW |
| BM527_05585 | 1299661..1300689 | + | 1029 | APD85601 | conjugal transfer protein TraU | traU |
| BM527_05590 | 1300692..1304384 | + | 3693 | APD85602 | conjugal transfer protein TraN | traN |
| BM527_05595 | 1304507..1305034 | - | 528 | APD85603 | hypothetical protein | - |
| BM527_05600 | 1305044..1305817 | - | 774 | APD85604 | hypothetical protein | - |
| BM527_05605 | 1306290..1307219 | + | 930 | APD88103 | DNA processing protein DprA | - |
Host bacterium
| ID | 465 | Element type | ICE (Integrative and conjugative element) |
| Element name | ICEAspMex1 | GenBank | CP018023 |
| Element size | 4489455 bp | Coordinate of oriT [Strand] | 3194..3492 [+] |
| Host bacterium | Alteromonas sp. Mex14 | Coordinate of element | 1253680..1337537 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | - |