Detailed information of oriT
oriT
The information of the oriT region
| oriTDB ID | 200514 |
| Name | oriT_TnGBS2.4  |
| Organism | Streptococcus agalactiae strain cz183 DDE transposase (TnGBS2.4_01c) relaxase (TnGBS2.4_02c) hypothetical protein |
| Sequence Completeness | - |
| NCBI accession of oriT (coordinates [strand]) | KC492041 (3446..3478 [-], 33 nt) |
| oriT length | 33 nt |
| IRs (inverted repeats) | _ |
| Location of nic site | _ |
| Conserved sequence flanking the nic site |
_ |
| Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 33 nt
>oriT_TnGBS2.4
GCATGTGTCCGGACACATGCTAATCTTGCCAGA
GCATGTGTCCGGACACATGCTAATCTTGCCAGA
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure file
Relaxase
| ID | 14570 | GenBank | AGO89396 |
| Name | Relaxase_TnGBS2.4_02c_TnGBS2.4 |
UniProt ID | _ |
| Length | 542 a.a. | PDB ID | |
| Note | Predicted by oriTfinder 2.0 | ||
Relaxase protein sequence
Download Length: 542 a.a. Molecular weight: 63623.19 Da Isoelectric Point: 8.9856
>AGO89396.1 relaxase [Streptococcus agalactiae]
MVVTKVIQIKSSKNLKRAINYITRDDATLIHDNGSQKEEDVFSYELVNNQVMKRLVAGHHLTDISDVDMI
YEDFILLKQSVDAFYDNEVLSDLKNDNRVLAHHVIQSFSPEDHLTPEQVNEIGRKTALELTGGHYQFVIA
THVDKGHLHNHIIFNTTNEVTLKKFRWQKNTARNLFQISNKHAEVYGAKILKPKMRNSYTDYSAWRRKNN
FRVEIKERLDFLMKHSLDQKDFFQKARALDLRIDTSRKYVTYQLTDQPQQRPVRDRTLSKKGKYSLEKLQ
ERFATNEVVYNLENVKDRYEEEKIKKEEDFELKITIEPWQITHLTAASIHIPITFGLDRKGTVSIPSRML
DQNDNGSFTAYLKRNDFFYFLNADHSEQNRFIKGTTLIKQLAAQNGELILTKNKNISKLDRLVEEFNFLA
INQVSNFKQFEILEGQFLAQLSETDQTLESLDNKIVYLNKLLGALLDYQNELVPSQISLHLLETSKINKE
MAPESLRKEIKELQIERSTLQEHRDRIVTDYDFANEMKQRYNANRKQSQTRL
MVVTKVIQIKSSKNLKRAINYITRDDATLIHDNGSQKEEDVFSYELVNNQVMKRLVAGHHLTDISDVDMI
YEDFILLKQSVDAFYDNEVLSDLKNDNRVLAHHVIQSFSPEDHLTPEQVNEIGRKTALELTGGHYQFVIA
THVDKGHLHNHIIFNTTNEVTLKKFRWQKNTARNLFQISNKHAEVYGAKILKPKMRNSYTDYSAWRRKNN
FRVEIKERLDFLMKHSLDQKDFFQKARALDLRIDTSRKYVTYQLTDQPQQRPVRDRTLSKKGKYSLEKLQ
ERFATNEVVYNLENVKDRYEEEKIKKEEDFELKITIEPWQITHLTAASIHIPITFGLDRKGTVSIPSRML
DQNDNGSFTAYLKRNDFFYFLNADHSEQNRFIKGTTLIKQLAAQNGELILTKNKNISKLDRLVEEFNFLA
INQVSNFKQFEILEGQFLAQLSETDQTLESLDNKIVYLNKLLGALLDYQNELVPSQISLHLLETSKINKE
MAPESLRKEIKELQIERSTLQEHRDRIVTDYDFANEMKQRYNANRKQSQTRL
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
| ID | 17187 | GenBank | AGO89400 |
| Name | t4cp2_TnGBS2.4_06c_TnGBS2.4 |
UniProt ID | _ |
| Length | 685 a.a. | PDB ID | _ |
| Note | Predicted by oriTfinder 2.0 | ||
T4CP protein sequence
Download Length: 685 a.a. Molecular weight: 78313.40 Da Isoelectric Point: 5.2735
>AGO89400.1 putative VirD4 domain protein [Streptococcus agalactiae]
MQVQKKPFFPYLLLSLVLAFATHRCYVLYSVAPEPDMTNLFSQYTYVFKNFFVSPYLYFDISPLALLSAL
VGFFIGMLFYLKVKLGGNYRHGEESGSARFATAQELQGFQDKKLSNNMIFSKQAMMGLFNKLLPFEWQLN
KNVLVVGLPGDGKTFTFVKPNLMQMNSSFIVTDPKGVLVREVGTMLEENGYQIKVFDLVNLTNSDMFNPF
KYMTSELDIDRITEALVEGTKRGDREGEDFWLQAKLLLNRALLGYLYFDSQVRGYEPNLSMVADLLRNLK
RPNEKKASPVEKLFKQLEKAMPGNYACRQWELFNSNFEAETRTSVLAIVATQYSIFDHEAVTNLIKSDTM
NMDTWNTEKTAVFVAISETNKAYSFLVSTFFTVVFDQLTHQVDAIIQGQKKGYGPEDLLHVQFIFDEFAN
CGKIPHFNEVLSSIRSREMSIKIIIQAISQLDSLYGLPARKSIVNNCATLLFLGTNDEDTMKYFSMRAGK
QTIIQTSYSEQRGQRVSGTTSRQSHQRDLMTPDEIARIGVDEALVFITKQNVFRDKKARVTDHPMKDRLA
NHYKDDTWYDYKRFMEDGAEFIDAVMTGKIKEKQVWAPDMDNYQAFVESNGFNNQFSKVTESLVYEQVPV
TVPEMTSEGQSQALSTGENQFKIVDMKTGEIFELPPEDRADAFGEVSLNDERLDV
MQVQKKPFFPYLLLSLVLAFATHRCYVLYSVAPEPDMTNLFSQYTYVFKNFFVSPYLYFDISPLALLSAL
VGFFIGMLFYLKVKLGGNYRHGEESGSARFATAQELQGFQDKKLSNNMIFSKQAMMGLFNKLLPFEWQLN
KNVLVVGLPGDGKTFTFVKPNLMQMNSSFIVTDPKGVLVREVGTMLEENGYQIKVFDLVNLTNSDMFNPF
KYMTSELDIDRITEALVEGTKRGDREGEDFWLQAKLLLNRALLGYLYFDSQVRGYEPNLSMVADLLRNLK
RPNEKKASPVEKLFKQLEKAMPGNYACRQWELFNSNFEAETRTSVLAIVATQYSIFDHEAVTNLIKSDTM
NMDTWNTEKTAVFVAISETNKAYSFLVSTFFTVVFDQLTHQVDAIIQGQKKGYGPEDLLHVQFIFDEFAN
CGKIPHFNEVLSSIRSREMSIKIIIQAISQLDSLYGLPARKSIVNNCATLLFLGTNDEDTMKYFSMRAGK
QTIIQTSYSEQRGQRVSGTTSRQSHQRDLMTPDEIARIGVDEALVFITKQNVFRDKKARVTDHPMKDRLA
NHYKDDTWYDYKRFMEDGAEFIDAVMTGKIKEKQVWAPDMDNYQAFVESNGFNNQFSKVTESLVYEQVPV
TVPEMTSEGQSQALSTGENQFKIVDMKTGEIFELPPEDRADAFGEVSLNDERLDV
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 10651..24146
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| TnGBS2.4_06c | 8594..10651 | - | 2058 | AGO89400 | putative VirD4 domain protein | - |
| TnGBS2.4_07c | 10651..11142 | - | 492 | AGO89401 | hypothetical protein | cd411 |
| TnGBS2.4_08c | 11167..11937 | - | 771 | AGO89402 | hypothetical protein | - |
| TnGBS2.4_09c | 12227..12838 | - | 612 | AGO89403 | hypothetical protein | prgL |
| TnGBS2.4_10c | 12855..15146 | - | 2292 | AGO89404 | VirB1 domain protein | prgK |
| TnGBS2.4_11c | 15584..17992 | - | 2409 | AGO89405 | VirB4 domain protein | virb4 |
| TnGBS2.4_12c | 17922..18275 | - | 354 | AGO89406 | hypothetical protein | - |
| TnGBS2.4_13c | 18347..18925 | - | 579 | AGO89407 | hypothetical protein | - |
| TnGBS2.4_14c | 18909..19367 | - | 459 | AGO89408 | hypothetical protein | - |
| TnGBS2.4_15c | 19378..20130 | - | 753 | AGO89409 | putative VirB6 domain protein | prgHb |
| TnGBS2.4_16c | 20184..20729 | - | 546 | AGO89410 | hypothetical protein | - |
| TnGBS2.4_17c | 21146..23347 | - | 2202 | AGO89411 | LPXTG protein | - |
| TnGBS2.4_18c | 23367..24146 | - | 780 | AGO89412 | LPXTG protein | prgC |
| TnGBS2.4_19c | 24149..24481 | - | 333 | AGO89413 | hypothetical protein | - |
| TnGBS2.4_20c | 24775..25101 | - | 327 | AGO89414 | putative single-strand DNA binding protein | - |
| TnGBS2.4_22c | 25843..26496 | - | 654 | AGO89415 | hypothetical protein | - |
| TnGBS2.4_23c | 26399..26890 | - | 492 | AGO89416 | hypothetical protein | - |
| TnGBS2.4_24c | 27152..27277 | - | 126 | AGO89417 | hypothetical protein | - |
| TnGBS2.4_25 | 27707..28108 | + | 402 | AGO89419 | hypothetical protein | - |
| TnGBS2.4_26c | 28644..29015 | - | 372 | AGO89418 | hypothetical protein | - |
Host bacterium
| ID | 457 | Element type | ICE (Integrative and conjugative element) |
| Element name | TnGBS2.4 | GenBank | KC492041 |
| Element size | 29151 bp | Coordinate of oriT [Strand] | 3446..3478 [-] |
| Host bacterium | Streptococcus agalactiae strain cz183 DDE transposase (TnGBS2.4_01c) relaxase (TnGBS2.4_02c) hypothetical protein | Coordinate of element | 358..29015 |
Cargo genes
| Drug resistance gene | - |
| Virulence gene | - |
| Metal resistance gene | - |
| Degradation gene | - |
| Symbiosis gene | - |
| Anti-CRISPR | AcrIIA21 |