Detailed information of oriT

oriT

The information of the oriT region


oriTDB ID   200510
Name   oriT2_Tn1549-like in_silico
Organism   Clostridioides difficile strain AI0499 vancomycin resistance protein-like
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   KU558763 ( 27107..27134 [+], 28 nt)
oriT length   28 nt
IRs (inverted repeats)      1..7, 12..18  (GGGAGTG..CACTCCC)
Location of nic site      _
Conserved sequence flanking the
  nic site    		  _
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 28 nt

>oriT2_Tn1549-like
GGGAGTGGCTACACTCCCTATGCTTGCT

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase

ID   14563 GenBank   ANW86175
Name   Relaxase_-_Tn1549-like insolico UniProt ID   _
Length   325 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 325 a.a.        Molecular weight: 37191.76 Da        Isoelectric Point: 10.1573

>ANW86175.1 putative endonuclease relaxase-like protein [Clostridioides difficile]
MVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSYAEWDAQRKGTSWKAKLKAAIDAAIPQA
KDFDDFLRLMQAQGYEIKPGKFISFRAPGQERFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLR
IDLENNIKAQQSAGYERWAKLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRL
ADMAVLIKNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALKAEYKR
LAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL

  Protein domains


Predicted by InterproScan.

Relaxase/Mobilisation nuclease domain (Relaxase)

(6-123)


  Protein structure



No available structure.




Auxiliary protein

ID   7933 GenBank   ANW86173
Name   ANW86173_Tn1549-like insolico UniProt ID   _
Length   109 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 109 a.a.        Molecular weight: 12486.63 Da        Isoelectric Point: 10.5610

>ANW86173.1 hypothetical protein [Clostridioides difficile]
MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLIDGYIVNMDTSDIRAQTAEIQKIGVNVN
QIAKRLNGMGPVYAQDIEDIKGALAQIWQLQRYILSSQR

  Protein domains


Predicted by InterproScan.

Bacterial mobilisation protein (MobC) (MobC)

(60-99)


  Protein structure



No available structure.




T4CP

ID   17180 GenBank   ANW86186
Name   t4cp2_-_Tn1549-like insolico UniProt ID   _
Length   424 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 424 a.a.        Molecular weight: 48212.49 Da        Isoelectric Point: 4.5883

>ANW86186.1 putative conjugative transfer protein-like protein [Clostridioides difficile]
MSRVSFYTRPYDRLEPYDGKLSRTVLRGGTGSNASLLPDIHSEKDVLKLVNTLISNTKGEGEKSAEDFWV
KSERLFYSALIGYIWYEAPAEEMNFSTLLEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKK
FLLSAGKTRSSILISCGARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQ
LINLLCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLKAIYKDAAE
IISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNYQKLGKELMSQDEIAVMDGGK
CILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDVDRFLTITRRKRQQVVTQDEVFDLYEIDLSDED
AAAE

  Protein domains


Predicted by InterproScan.

Type IV secretory system Conjugative DNA transfer (T4SS-DNA_transf)

(41-365)

  Protein structure



No available structure.




Host bacterium

ID   453 Element type   ICE (Integrative and conjugative element)
Element name   Tn1549-like GenBank   KU558763
Element size   42375 bp Coordinate of oriT [Strand]   16604..16633 [-]; 27107..27134 [+]
Host bacterium   Clostridioides difficile strain AI0499 vancomycin resistance protein-like Coordinate of element   1..42375

Cargo genes

Drug resistance gene   VanHBX
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   -