Detailed information of oriT

oriT


The information of the oriT region


oriTDB ID   200507
Name   oriT_ICESsuJH1308-2 in_silico
Organism   Streptococcus suis integrative and
Sequence Completeness      -
NCBI accession of oriT (coordinates [strand])   KX077884 (44867..44999 [+], 133 nt)
oriT length   133 nt
IRs (inverted repeats)      65..70, 83..88  (AAATCC..GGATTT)
 6..12, 23..29  (ACCCCCC..GGGGGGT)
Location of nic site      75..76
Conserved sequence flanking the
  nic site  
 
 TTTGGTTACA
Note   Predicted by oriTfinder 2.0

  oriT sequence  


Download         Length: 133 nt

>oriT_ICESsuJH1308-2
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC

Visualization of oriT structure

  oriT secondary structure

Predicted by RNAfold.

Download structure file


Relaxase


ID   14558 GenBank   ANJ64179
Name   Rep_trans_-_ICESsuJH1308-2 insolico UniProt ID   _
Length   401 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 401 a.a.        Molecular weight: 47388.06 Da        Isoelectric Point: 6.5091

>ANJ64179.1 putative transcriptional regulator [Streptococcus suis]
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKSSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK

  Protein domains


Predicted by InterproScan.

(15-49)

(169-373)

(73-161)


  Protein structure



No available structure.



ID   14559 GenBank   ANJ64197
Name   Relaxase_-_ICESsuJH1308-2 insolico UniProt ID   _
Length   621 a.a. PDB ID   
Note   Predicted by oriTfinder 2.0

  Relaxase protein sequence


Download         Length: 621 a.a.        Molecular weight: 73793.64 Da        Isoelectric Point: 7.0198

>ANJ64197.1 relaxase [Streptococcus suis]
MVITKHYATHSRKYRKNLIRYILNPEKTAQLKLVSDFGMSNYLDFPSYEEMVKMYQANFLNNDGLYDSRN
DRQEKRQQKIHAHHLIQSFSPDDKLTPEEINRIGYETVKELTGGNFRFIVATHIDQSHIHNHILINSVDL
QSDKKLKWNYALERNLRMISDRISKEAGAKIITNRYSHQQYDVYRKTNHKFELKQRLYFLMEQSKDFEDF
LKKAEQLHLTIDFSSKHTTFLMTDRDQVKPIRGRQLNRREPYDESYFRQAFAKGAIEQRLHFLLPRVKTL
QELLEMAEELGLFIQLKQKNVTFTLTENDQKISLGHQQVSKKKLYDVSFFQDYFSERSDTDIELSDNIKE
DFEIFLAEQAEKLSTVESIVSDYETFSENRKAVHEFEVELAPHQVDKEVEGGIFIKVAFGVKKEGLIFIP
NYQLDEIEKEEEKRFKIFLREITSYFIYNKESSDKNRYMKGRDLIRQLTQDNKSLPYRRRPNLKELQEKI
TEVNLLIELNVTNRAYTDIKDELVAEIATYDVSMSELNEKTATLNKIAEVLVNLKSHDIERRRLARYEFS
KLNLTESVTLEQIEREISNTQDQLSKLLDNYEESVRKLETFVAVLNRTAPRTRKDYDKELY

  Protein domains


Predicted by InterproScan.

(13-261)

(517-608)


  Protein structure



No available structure.




Auxiliary protein


ID   7930 GenBank   ANJ64193
Name   ANJ64193_ICESsuJH1308-2 insolico UniProt ID   _
Length   405 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  Auxiliary protein sequence


Download         Length: 405 a.a.        Molecular weight: 47021.92 Da        Isoelectric Point: 9.7978

>ANJ64193.1 integrase [Streptococcus suis]
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA

  Protein domains


Predicted by InterproScan.

(190-383)

(1-71)


  Protein structure



No available structure.




T4CP


ID   17175 GenBank   ANJ64142
Name   t4cp2_-_ICESsuJH1308-2 insolico UniProt ID   _
Length   605 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 605 a.a.        Molecular weight: 69397.18 Da        Isoelectric Point: 9.2929

>ANJ64142.1 hypothetical protein [Streptococcus suis]
MYSRRKAFVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLWLFAFTQKSLLAFIL
GVLTMTLVYLYVSTGQKVYREGEEYGSARFGTSKEKRNFYSKNPFNDTILARDVRLTLLEKKKPLFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNI
QSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQADVDFKGQLPIHVRSYL
DEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFM
SGLLGKQTIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHK
NWKWLADKETDERWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH

  Protein domains


Predicted by InterproScan.

(135-555)

  Protein structure



No available structure.



ID   17176 GenBank   ANJ64177
Name   tcpA_-_ICESsuJH1308-2 insolico UniProt ID   _
Length   461 a.a. PDB ID   _
Note   Predicted by oriTfinder 2.0

  T4CP protein sequence


Download         Length: 461 a.a.        Molecular weight: 53414.28 Da        Isoelectric Point: 8.9454

>ANJ64177.1 hypothetical protein [Streptococcus suis]
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEDLLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD

  Protein domains


Predicted by InterproScan.

(217-301)

  Protein structure



No available structure.




T4SS


T4SS were predicted by using oriTfinder2.

Region 1: 3042..54463

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Locus_0 1..366 + 366 ANJ64212 LSU ribosomal protein L7/L12 (P1/P2) -
Locus_1 386..505 + 120 ANJ64132 hypothetical protein -
Locus_2 565..738 + 174 ANJ64133 hypothetical protein -
Locus_3 735..1277 + 543 ANJ64134 hypothetical protein -
Locus_4 1243..1554 + 312 ANJ64135 hypothetical protein -
Locus_5 1703..3058 + 1356 ANJ64136 modification methylase -
Locus_6 3042..3470 + 429 ANJ64137 hypothetical protein gbs1369
Locus_7 3481..3864 + 384 ANJ64138 hypothetical protein -
Locus_8 3873..4106 + 234 ANJ64139 hypothetical protein -
Locus_9 4109..4693 + 585 ANJ64140 hypothetical protein -
Locus_10 4771..5259 + 489 ANJ64141 hypothetical protein gbs1365
Locus_11 5259..7076 + 1818 ANJ64142 hypothetical protein virb4
Locus_12 7094..7336 + 243 ANJ64143 hypothetical protein prgF
Locus_13 7355..8209 + 855 ANJ64144 hypothetical protein prgHb
Locus_14 8271..8624 + 354 ANJ64145 hypothetical protein prgIc
Locus_15 8581..10932 + 2352 ANJ64146 TrsE-like protein virb4
Locus_16 10934..13735 + 2802 ANJ64147 hypothetical protein prgK
Locus_17 13796..14641 - 846 ANJ64148 abortive infection protein AbiGII -
Locus_18 14638..15228 - 591 ANJ64149 abortive infection protein AbiGI -
Locus_19 15365..20296 + 4932 ANJ64150 agglutinin receptor prgB
Locus_20 20297..20488 + 192 ANJ64151 putative calcium-binding protein -
Locus_21 20472..21023 + 552 ANJ64152 hypothetical protein gbs1354
Locus_22 21073..27897 + 6825 ANJ64153 SNF2 family protein -
Locus_23 27968..28267 + 300 ANJ64154 hypothetical protein -
Locus_24 28281..28577 + 297 ANJ64155 hypothetical protein gbs1350
Locus_25 28918..29091 + 174 ANJ64156 hypothetical protein -
Locus_26 29111..29290 - 180 ANJ64157 hypothetical protein -
Locus_27 29709..29828 - 120 ANJ64158 hypothetical protein -
Locus_28 29905..30756 - 852 ANJ64159 putative aminoglycoside 6-adenylyltansferase -
Locus_29 30789..31523 - 735 ANJ64160 ubiquinone/menaquinone biosynthesis -
Locus_30 31504..32373 - 870 ANJ64161 hypothetical protein -
Locus_31 32388..32612 - 225 ANJ64162 helix-turn-helix domain protein -
Locus_32 34194..34328 - 135 ANJ64163 hypothetical protein -
Locus_33 34327..34965 + 639 ANJ64164 hypothetical protein prgL
Locus_34 35004..36080 + 1077 ANJ64165 hypothetical protein -
Locus_35 36134..36361 + 228 ANJ64166 hypothetical protein gbs1347
Locus_36 36358..36747 + 390 ANJ64167 hypothetical protein gbs1346
Locus_37 36797..37087 + 291 ANJ64168 hypothetical protein -
Locus_38 37157..37633 + 477 ANJ64169 transcription regulator, probable -
Locus_39 37633..38409 + 777 ANJ64170 zeta toxin -
Locus_40 38406..40493 + 2088 ANJ64171 putative ATP-dependent endonuclease -
Locus_41 40480..42297 + 1818 ANJ64172 UvrD/REP helicase family protein -
Locus_42 42392..42691 - 300 ANJ64173 Abi-alpha protein -
Locus_43 42911..43030 + 120 ANJ64174 hypothetical protein -
Locus_44 43053..43367 + 315 ANJ64175 hypothetical protein orf23
Locus_45 43386..43769 + 384 ANJ64176 hypothetical protein orf23
Locus_46 43798..45183 + 1386 ANJ64177 hypothetical protein virb4
Locus_47 45186..45338 + 153 ANJ64178 hypothetical protein -
Locus_48 45361..46566 + 1206 ANJ64179 putative transcriptional regulator -
Locus_49 46609..46830 + 222 ANJ64180 conjugation related protein orf19
Locus_50 46947..47444 + 498 ANJ64181 antirestriction protein -
Locus_51 47533..47925 + 393 ANJ64182 hypothetical protein orf17a
Locus_52 47909..50356 + 2448 ANJ64183 hypothetical protein virb4
Locus_53 50425..52536 + 2112 ANJ64184 putative membrane protein orf15
Locus_54 52533..53534 + 1002 ANJ64185 NLP/P60 family lipoprotein orf14
Locus_55 53531..54463 + 933 ANJ64186 hypothetical protein orf13
Locus_56 54840..56759 + 1920 ANJ64187 tetracycline resistance protein TetM -
Locus_57 57105..57458 - 354 ANJ64188 hypothetical protein -
Locus_58 57963..58385 + 423 ANJ64189 hypothetical protein -
Locus_59 58382..58612 + 231 ANJ64190 hypothetical protein -
Locus_60 58676..58804 - 129 ANJ64191 hypothetical protein -
Locus_61 59073..59276 + 204 ANJ64192 Xis-Tn protein -


Host bacterium


ID   451 Element type   ICE (Integrative and conjugative element)
Element name   ICESsuJH1308-2 GenBank   KX077884
Element size   76902 bp Coordinate of oriT [Strand]   44867..44999 [+]
Host bacterium   Streptococcus suis integrative and Coordinate of element   352..76028

Cargo genes


Drug resistance gene   ant(6)-Ia, tet(M)
Virulence gene   -
Metal resistance gene   -
Degradation gene   -
Symbiosis gene   -
Anti-CRISPR   AcrIIA8, AcrIIA21