Detailed information of oriT
oriT
The information of the oriT region
oriTDB ID | 200507 |
Name | oriT_ICESsuJH1308-2 |
Organism | Streptococcus suis integrative and |
Sequence Completeness | - |
NCBI accession of oriT (coordinates [strand]) | KX077884 (44867..44999 [+], 133 nt) |
oriT length | 133 nt |
IRs (inverted repeats) | 65..70, 83..88 (AAATCC..GGATTT) 6..12, 23..29 (ACCCCCC..GGGGGGT) |
Location of nic site | 75..76 |
Conserved sequence flanking the nic site |
TTTGGTTACA |
Note | Predicted by oriTfinder 2.0 |
oriT sequence
Download Length: 133 nt
ACTTAACCCCCCGTATCTAACAGGGGGGTACAAATCGACAGGAAACAGTCAAAAAAACATTAGAAAATCCTTTGGTTACAAGGGATTTACAAAATTTCAGCGTATGTCAAATGGGCTTTAAAAGTTGACATAC
Visualization of oriT structure
oriT secondary structure
Predicted by RNAfold.
Download structure fileRelaxase
ID | 14558 | GenBank | ANJ64179 |
Name | Rep_trans_-_ICESsuJH1308-2 | UniProt ID | _ |
Length | 401 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 401 a.a. Molecular weight: 47388.06 Da Isoelectric Point: 6.5091
MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIETGKVKSSEDLQQSLWEALERFNPDAP
LEMLFDYVRIRFPTTDVQQVVENILQLKLSYFLHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGR
GCRQFESYLLAQQRSWYEFFMDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRS
GELVRKEEKECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAVRDLL
VYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKPEPYSFQRTLNWLSHQV
APTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVKEQDVITTKK
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 14559 | GenBank | ANJ64197 |
Name | Relaxase_-_ICESsuJH1308-2 | UniProt ID | _ |
Length | 621 a.a. | PDB ID | |
Note | Predicted by oriTfinder 2.0 |
Relaxase protein sequence
Download Length: 621 a.a. Molecular weight: 73793.64 Da Isoelectric Point: 7.0198
MVITKHYATHSRKYRKNLIRYILNPEKTAQLKLVSDFGMSNYLDFPSYEEMVKMYQANFLNNDGLYDSRN
DRQEKRQQKIHAHHLIQSFSPDDKLTPEEINRIGYETVKELTGGNFRFIVATHIDQSHIHNHILINSVDL
QSDKKLKWNYALERNLRMISDRISKEAGAKIITNRYSHQQYDVYRKTNHKFELKQRLYFLMEQSKDFEDF
LKKAEQLHLTIDFSSKHTTFLMTDRDQVKPIRGRQLNRREPYDESYFRQAFAKGAIEQRLHFLLPRVKTL
QELLEMAEELGLFIQLKQKNVTFTLTENDQKISLGHQQVSKKKLYDVSFFQDYFSERSDTDIELSDNIKE
DFEIFLAEQAEKLSTVESIVSDYETFSENRKAVHEFEVELAPHQVDKEVEGGIFIKVAFGVKKEGLIFIP
NYQLDEIEKEEEKRFKIFLREITSYFIYNKESSDKNRYMKGRDLIRQLTQDNKSLPYRRRPNLKELQEKI
TEVNLLIELNVTNRAYTDIKDELVAEIATYDVSMSELNEKTATLNKIAEVLVNLKSHDIERRRLARYEFS
KLNLTESVTLEQIEREISNTQDQLSKLLDNYEESVRKLETFVAVLNRTAPRTRKDYDKELY
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
Auxiliary protein
ID | 7930 | GenBank | ANJ64193 |
Name | ANJ64193_ICESsuJH1308-2 | UniProt ID | _ |
Length | 405 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
Auxiliary protein sequence
Download Length: 405 a.a. Molecular weight: 47021.92 Da Isoelectric Point: 9.7978
MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKLVATDRVPAGKRDCISLREKIAELQKD
IHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNTETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSE
NGYAYQTINNYKRSLKASFYIAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYS
KNYDEILILLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVEEAYQ
AFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNEDKLPHITPHSLRHTFC
TNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMKRLNKEKQQERLVA
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4CP
ID | 17175 | GenBank | ANJ64142 |
Name | t4cp2_-_ICESsuJH1308-2 | UniProt ID | _ |
Length | 605 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 605 a.a. Molecular weight: 69397.18 Da Isoelectric Point: 9.2929
MYSRRKAFVFGLLGLAFGYFCHRLTLLYDSLTNAPPMERFAYLLGEGLNQVFNPLWLFAFTQKSLLAFIL
GVLTMTLVYLYVSTGQKVYREGEEYGSARFGTSKEKRNFYSKNPFNDTILARDVRLTLLEKKKPLFDRNK
NLIVIGGSGAGKTFRFVKPNLIQLNCSNIVVDPKDHLAEKTGKLFLENGYQVKVLDLVNMTNSDGFNPFR
YVETENDLNRMLTVYFNNTRGSGSRSDPFWDEASMTLVRAIASYLVDFYNPPGSSKQEQEARRKRGRYPA
FSEIGKLIKLLSKGDNQDKSVLEVLFEDYAKKYGHENFTMRNWADFQNYKDKTLDSVIAVTTAKFALFNI
QSVIDLTQRDTMDLKTWGTQKTMVYLVIPDNDTTFRFLSALFFSTVFSTLTRQADVDFKGQLPIHVRSYL
DEFANVGEIPDFAEQTSTVRSRNMSLVPILQNIAQLQGLYKEKEAWKTILGNCDSLLYLGGNDEETFKFM
SGLLGKQTIDVRSTSRSFGQTGSSSTSHQKIARDLMTADEVGNMKRDECLVRIAGVPVFRTKKYFPLKHK
NWKWLADKETDERWWHYHINPLTAEEEVDLSGHKIRDLSTETTLH
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
ID | 17176 | GenBank | ANJ64177 |
Name | tcpA_-_ICESsuJH1308-2 | UniProt ID | _ |
Length | 461 a.a. | PDB ID | _ |
Note | Predicted by oriTfinder 2.0 |
T4CP protein sequence
Download Length: 461 a.a. Molecular weight: 53414.28 Da Isoelectric Point: 8.9454
MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQINWQDFNLSQADKIDIPYLIISFSVAIL
ICLLVAFVFKRVRYDTVKQLYHRQKLAKMILENKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKN
GLIQIRVEITLGKYQDQLLHLEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRL
MKNVWWEYDKLPHMLIAGGTGGGKTYFILTLIEDLLHTDSKLYILDPKNADLADLGSVMANVYYRKEDLL
SCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTKENTAVMNKLKQIVMLG
RQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYGMMFGSDVQKDFFLKRIKGRGYVDVGTSV
ISEFYTPLVPKGYDFLEEIKKLSNSRQSTQATCEAEVAGVD
Protein domains
Predicted by InterproScan.
Protein structure
No available structure.
T4SS
T4SS were predicted by using oriTfinder2.
Region 1: 3042..54463
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
Locus_0 | 1..366 | + | 366 | ANJ64212 | LSU ribosomal protein L7/L12 (P1/P2) | - |
Locus_1 | 386..505 | + | 120 | ANJ64132 | hypothetical protein | - |
Locus_2 | 565..738 | + | 174 | ANJ64133 | hypothetical protein | - |
Locus_3 | 735..1277 | + | 543 | ANJ64134 | hypothetical protein | - |
Locus_4 | 1243..1554 | + | 312 | ANJ64135 | hypothetical protein | - |
Locus_5 | 1703..3058 | + | 1356 | ANJ64136 | modification methylase | - |
Locus_6 | 3042..3470 | + | 429 | ANJ64137 | hypothetical protein | gbs1369 |
Locus_7 | 3481..3864 | + | 384 | ANJ64138 | hypothetical protein | - |
Locus_8 | 3873..4106 | + | 234 | ANJ64139 | hypothetical protein | - |
Locus_9 | 4109..4693 | + | 585 | ANJ64140 | hypothetical protein | - |
Locus_10 | 4771..5259 | + | 489 | ANJ64141 | hypothetical protein | gbs1365 |
Locus_11 | 5259..7076 | + | 1818 | ANJ64142 | hypothetical protein | virb4 |
Locus_12 | 7094..7336 | + | 243 | ANJ64143 | hypothetical protein | prgF |
Locus_13 | 7355..8209 | + | 855 | ANJ64144 | hypothetical protein | prgHb |
Locus_14 | 8271..8624 | + | 354 | ANJ64145 | hypothetical protein | prgIc |
Locus_15 | 8581..10932 | + | 2352 | ANJ64146 | TrsE-like protein | virb4 |
Locus_16 | 10934..13735 | + | 2802 | ANJ64147 | hypothetical protein | prgK |
Locus_17 | 13796..14641 | - | 846 | ANJ64148 | abortive infection protein AbiGII | - |
Locus_18 | 14638..15228 | - | 591 | ANJ64149 | abortive infection protein AbiGI | - |
Locus_19 | 15365..20296 | + | 4932 | ANJ64150 | agglutinin receptor | prgB |
Locus_20 | 20297..20488 | + | 192 | ANJ64151 | putative calcium-binding protein | - |
Locus_21 | 20472..21023 | + | 552 | ANJ64152 | hypothetical protein | gbs1354 |
Locus_22 | 21073..27897 | + | 6825 | ANJ64153 | SNF2 family protein | - |
Locus_23 | 27968..28267 | + | 300 | ANJ64154 | hypothetical protein | - |
Locus_24 | 28281..28577 | + | 297 | ANJ64155 | hypothetical protein | gbs1350 |
Locus_25 | 28918..29091 | + | 174 | ANJ64156 | hypothetical protein | - |
Locus_26 | 29111..29290 | - | 180 | ANJ64157 | hypothetical protein | - |
Locus_27 | 29709..29828 | - | 120 | ANJ64158 | hypothetical protein | - |
Locus_28 | 29905..30756 | - | 852 | ANJ64159 | putative aminoglycoside 6-adenylyltansferase | - |
Locus_29 | 30789..31523 | - | 735 | ANJ64160 | ubiquinone/menaquinone biosynthesis | - |
Locus_30 | 31504..32373 | - | 870 | ANJ64161 | hypothetical protein | - |
Locus_31 | 32388..32612 | - | 225 | ANJ64162 | helix-turn-helix domain protein | - |
Locus_32 | 34194..34328 | - | 135 | ANJ64163 | hypothetical protein | - |
Locus_33 | 34327..34965 | + | 639 | ANJ64164 | hypothetical protein | prgL |
Locus_34 | 35004..36080 | + | 1077 | ANJ64165 | hypothetical protein | - |
Locus_35 | 36134..36361 | + | 228 | ANJ64166 | hypothetical protein | gbs1347 |
Locus_36 | 36358..36747 | + | 390 | ANJ64167 | hypothetical protein | gbs1346 |
Locus_37 | 36797..37087 | + | 291 | ANJ64168 | hypothetical protein | - |
Locus_38 | 37157..37633 | + | 477 | ANJ64169 | transcription regulator, probable | - |
Locus_39 | 37633..38409 | + | 777 | ANJ64170 | zeta toxin | - |
Locus_40 | 38406..40493 | + | 2088 | ANJ64171 | putative ATP-dependent endonuclease | - |
Locus_41 | 40480..42297 | + | 1818 | ANJ64172 | UvrD/REP helicase family protein | - |
Locus_42 | 42392..42691 | - | 300 | ANJ64173 | Abi-alpha protein | - |
Locus_43 | 42911..43030 | + | 120 | ANJ64174 | hypothetical protein | - |
Locus_44 | 43053..43367 | + | 315 | ANJ64175 | hypothetical protein | orf23 |
Locus_45 | 43386..43769 | + | 384 | ANJ64176 | hypothetical protein | orf23 |
Locus_46 | 43798..45183 | + | 1386 | ANJ64177 | hypothetical protein | virb4 |
Locus_47 | 45186..45338 | + | 153 | ANJ64178 | hypothetical protein | - |
Locus_48 | 45361..46566 | + | 1206 | ANJ64179 | putative transcriptional regulator | - |
Locus_49 | 46609..46830 | + | 222 | ANJ64180 | conjugation related protein | orf19 |
Locus_50 | 46947..47444 | + | 498 | ANJ64181 | antirestriction protein | - |
Locus_51 | 47533..47925 | + | 393 | ANJ64182 | hypothetical protein | orf17a |
Locus_52 | 47909..50356 | + | 2448 | ANJ64183 | hypothetical protein | virb4 |
Locus_53 | 50425..52536 | + | 2112 | ANJ64184 | putative membrane protein | orf15 |
Locus_54 | 52533..53534 | + | 1002 | ANJ64185 | NLP/P60 family lipoprotein | orf14 |
Locus_55 | 53531..54463 | + | 933 | ANJ64186 | hypothetical protein | orf13 |
Locus_56 | 54840..56759 | + | 1920 | ANJ64187 | tetracycline resistance protein TetM | - |
Locus_57 | 57105..57458 | - | 354 | ANJ64188 | hypothetical protein | - |
Locus_58 | 57963..58385 | + | 423 | ANJ64189 | hypothetical protein | - |
Locus_59 | 58382..58612 | + | 231 | ANJ64190 | hypothetical protein | - |
Locus_60 | 58676..58804 | - | 129 | ANJ64191 | hypothetical protein | - |
Locus_61 | 59073..59276 | + | 204 | ANJ64192 | Xis-Tn protein | - |
Host bacterium
ID | 451 | Element type | ICE (Integrative and conjugative element) |
Element name | ICESsuJH1308-2 | GenBank | KX077884 |
Element size | 76902 bp | Coordinate of oriT [Strand] | 44867..44999 [+] |
Host bacterium | Streptococcus suis integrative and | Coordinate of element | 352..76028 |
Cargo genes
Drug resistance gene | ant(6)-Ia, tet(M) |
Virulence gene | - |
Metal resistance gene | - |
Degradation gene | - |
Symbiosis gene | - |
Anti-CRISPR | AcrIIA8, AcrIIA21 |